Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae.
Publication
, Journal Article
Schiestl, RH; Petes, TD
Published in: Proc Natl Acad Sci U S A
September 1, 1991
DNA fragments (generated by BamHI treatment) with no homology to the yeast genome were transformed into Saccharomyces cerevisiae. When the fragments were transformed in the presence of the BamHI enzyme, they integrated into genomic BamHI sites. When the fragments were transformed in the absence of the enzyme, they integrated into genomic G-A-T-C sites. Since the G-A-T-C sequence is present at the ends of BamHI fragments, this results indicates that four base pairs of homology are sufficient for some types of mitotic recombination.
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Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
September 1, 1991
Volume
88
Issue
17
Start / End Page
7585 / 7589
Location
United States
Related Subject Headings
- Transformation, Genetic
- Saccharomyces cerevisiae
- Restriction Mapping
- Recombination, Genetic
- Plasmids
- Open Reading Frames
- Molecular Sequence Data
- Models, Genetic
- Deoxyribonuclease BamHI
- DNA, Fungal
Citation
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Chicago
ICMJE
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NLM
Schiestl, R. H., & Petes, T. D. (1991). Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A, 88(17), 7585–7589. https://doi.org/10.1073/pnas.88.17.7585
Schiestl, R. H., and T. D. Petes. “Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae.” Proc Natl Acad Sci U S A 88, no. 17 (September 1, 1991): 7585–89. https://doi.org/10.1073/pnas.88.17.7585.
Schiestl RH, Petes TD. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7585–9.
Schiestl, R. H., and T. D. Petes. “Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae.” Proc Natl Acad Sci U S A, vol. 88, no. 17, Sept. 1991, pp. 7585–89. Pubmed, doi:10.1073/pnas.88.17.7585.
Schiestl RH, Petes TD. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7585–7589.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
September 1, 1991
Volume
88
Issue
17
Start / End Page
7585 / 7589
Location
United States
Related Subject Headings
- Transformation, Genetic
- Saccharomyces cerevisiae
- Restriction Mapping
- Recombination, Genetic
- Plasmids
- Open Reading Frames
- Molecular Sequence Data
- Models, Genetic
- Deoxyribonuclease BamHI
- DNA, Fungal