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Most of the yeast genomic sequences are not essential for cell growth and division.

Publication ,  Journal Article
Goebl, MG; Petes, TD
Published in: Cell
September 26, 1986

To determine the fraction of the yeast Saccharomyces cerevisiae genome that is required for normal cell growth and division, we constructed diploid strains that were heterozygous for random single disruptions. We monitored the effects of approximately 200 independent disruptions by sporulating the diploids and examining the phenotype of the resulting haploid strains. We found that only 12% of the disruptions were haploid-lethal, 14% resulted in slow growth, and an additional 4% were associated with some other new phenotype (such as an auxotrophic requirement). No obvious new phenotype was detected for 70% of the disruptions.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 26, 1986

Volume

46

Issue

7

Start / End Page

983 / 992

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Phenotype
  • Mutation
  • Heterozygote
  • Genetic Engineering
  • Genes, Lethal
  • Genes, Fungal
  • Developmental Biology
  • DNA, Fungal
  • DNA Transposable Elements
 

Citation

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Goebl, M. G., & Petes, T. D. (1986). Most of the yeast genomic sequences are not essential for cell growth and division. Cell, 46(7), 983–992. https://doi.org/10.1016/0092-8674(86)90697-5
Goebl, M. G., and T. D. Petes. “Most of the yeast genomic sequences are not essential for cell growth and division.Cell 46, no. 7 (September 26, 1986): 983–92. https://doi.org/10.1016/0092-8674(86)90697-5.
Goebl, M. G., and T. D. Petes. “Most of the yeast genomic sequences are not essential for cell growth and division.Cell, vol. 46, no. 7, Sept. 1986, pp. 983–92. Pubmed, doi:10.1016/0092-8674(86)90697-5.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

September 26, 1986

Volume

46

Issue

7

Start / End Page

983 / 992

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Phenotype
  • Mutation
  • Heterozygote
  • Genetic Engineering
  • Genes, Lethal
  • Genes, Fungal
  • Developmental Biology
  • DNA, Fungal
  • DNA Transposable Elements