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TNF, IFN-gamma, and endotoxin increase expression of DMT1 in bronchial epithelial cells.

Publication ,  Journal Article
Wang, X; Garrick, MD; Yang, F; Dailey, LA; Piantadosi, CA; Ghio, AJ
Published in: Am J Physiol Lung Cell Mol Physiol
July 2005

Regulation of the metal transport protein divalent metal transporter-1 (DMT1) may contribute to the uptake and detoxification of iron by cells resident in the respiratory tract. Inflammation has been associated with an increased availability of this metal resulting in an oxidative stress. Because proinflammatory cytokines and LPS have been demonstrated to affect an elevated expression of DMT1 in a macrophage cell line, we tested the hypothesis that tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and LPS increase DMT1 expression in airway epithelial cells. We used RT-PCR to detect mRNA for both -IRE DMT1 and +IRE DMT1 in BEAS-2B cells. Treatment with TNF-alpha, IFN-gamma, or LPS increased both forms. Western blot analysis also demonstrated an increase in the expression of both isoforms of DMT1 after these treatments. Twenty-four hours after exposure of an animal model to TNF-alpha, IFN-gamma, or LPS, a significant increase in pulmonary expression of -IRE DMT1 was seen by immunohistochemistry; the level of +IRE DMT1 was too low in the lung to be visualized using this methodology. Finally, iron transport into BEAS-2B cells was increased after inclusion of TNF-alpha, IFN-gamma, or LPS in the media. We conclude that proinflammatory cytokines and LPS increase mRNA and protein expression of DMT1 in airway cells in vitro and in vivo. Furthermore, both -IRE and +IRE isoforms are elevated after exposures. Increased expression of this protein appears to be included in a coordinated response of the cell and tissue where the function might be to diminish availability of metal.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

July 2005

Volume

289

Issue

1

Start / End Page

L24 / L33

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Isoforms
  • Male
  • Lipopolysaccharides
  • Iron-Binding Proteins
  • Iron
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, X., Garrick, M. D., Yang, F., Dailey, L. A., Piantadosi, C. A., & Ghio, A. J. (2005). TNF, IFN-gamma, and endotoxin increase expression of DMT1 in bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol, 289(1), L24–L33. https://doi.org/10.1152/ajplung.00428.2003
Wang, Xinchao, Michael D. Garrick, Funmei Yang, Lisa A. Dailey, Claude A. Piantadosi, and Andrew J. Ghio. “TNF, IFN-gamma, and endotoxin increase expression of DMT1 in bronchial epithelial cells.Am J Physiol Lung Cell Mol Physiol 289, no. 1 (July 2005): L24–33. https://doi.org/10.1152/ajplung.00428.2003.
Wang X, Garrick MD, Yang F, Dailey LA, Piantadosi CA, Ghio AJ. TNF, IFN-gamma, and endotoxin increase expression of DMT1 in bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2005 Jul;289(1):L24–33.
Wang, Xinchao, et al. “TNF, IFN-gamma, and endotoxin increase expression of DMT1 in bronchial epithelial cells.Am J Physiol Lung Cell Mol Physiol, vol. 289, no. 1, July 2005, pp. L24–33. Pubmed, doi:10.1152/ajplung.00428.2003.
Wang X, Garrick MD, Yang F, Dailey LA, Piantadosi CA, Ghio AJ. TNF, IFN-gamma, and endotoxin increase expression of DMT1 in bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2005 Jul;289(1):L24–L33.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

July 2005

Volume

289

Issue

1

Start / End Page

L24 / L33

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Respiratory System
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Protein Isoforms
  • Male
  • Lipopolysaccharides
  • Iron-Binding Proteins
  • Iron