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Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus.

Publication ,  Journal Article
Pollara, JJ; Spesock, AH; Pickup, DJ; Laster, SM; Petty, ITD
Published in: Virology
July 5, 2012

Prostaglandin E₂ (PGE₂) is an arachidonic acid (AA)-derived signaling molecule that can influence host immune responses to infection or vaccination. In this study, we investigated PGE₂ production in vitro by cells infected with the poxvirus vaccine strain, modified vaccinia Ankara virus (MVA). Human THP-1 cells, murine bone marrow-derived dendritic cells, and murine C3HA fibroblasts all accumulated PGE₂ to high levels in culture supernatants upon infection with MVA. We also demonstrated that MVA induced the release of AA from infected cells, and this was, most unusually, independent of host cytosolic phospholipase A₂ activity. The accumulation of AA and PGE₂ was dependent on viral gene expression, but independent of canonical NF-κB signaling via p65/RelA. The production of PGE₂ required host cyclooxygenase-2 (COX-2) activity, and COX-2 protein accumulated during MVA infection. The results of this study provide insight into a novel aspect of MVA biology that may affect the efficacy of MVA-based vaccines.

Duke Scholars

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

July 5, 2012

Volume

428

Issue

2

Start / End Page

146 / 155

Location

United States

Related Subject Headings

  • Virology
  • Viral Proteins
  • Vaccinia virus
  • Vaccinia
  • Mice
  • Humans
  • Dinoprostone
  • Cyclooxygenase 2
  • Cell Line
  • Arachidonic Acid
 

Citation

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ICMJE
MLA
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Pollara, J. J., Spesock, A. H., Pickup, D. J., Laster, S. M., & Petty, I. T. D. (2012). Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus. Virology, 428(2), 146–155. https://doi.org/10.1016/j.virol.2012.03.019
Pollara, Justin J., April H. Spesock, David J. Pickup, Scott M. Laster, and Ian T. D. Petty. “Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus.Virology 428, no. 2 (July 5, 2012): 146–55. https://doi.org/10.1016/j.virol.2012.03.019.
Pollara JJ, Spesock AH, Pickup DJ, Laster SM, Petty ITD. Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus. Virology. 2012 Jul 5;428(2):146–55.
Pollara, Justin J., et al. “Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus.Virology, vol. 428, no. 2, July 2012, pp. 146–55. Pubmed, doi:10.1016/j.virol.2012.03.019.
Pollara JJ, Spesock AH, Pickup DJ, Laster SM, Petty ITD. Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus. Virology. 2012 Jul 5;428(2):146–155.
Journal cover image

Published In

Virology

DOI

EISSN

1096-0341

Publication Date

July 5, 2012

Volume

428

Issue

2

Start / End Page

146 / 155

Location

United States

Related Subject Headings

  • Virology
  • Viral Proteins
  • Vaccinia virus
  • Vaccinia
  • Mice
  • Humans
  • Dinoprostone
  • Cyclooxygenase 2
  • Cell Line
  • Arachidonic Acid