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Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens.

Publication ,  Journal Article
Cheng, QF; Rao, PV; Zigler, JS
Published in: Exp Eye Res
August 2001

Previous studies have demonstrated that agents affecting the cholesterol synthetic pathway can have cataractogenic effects. We have suggested that opacification of cultured lenses resulting from exposure to the cholesterol-lowering agent lovastatin is caused by inhibition of isoprenylation of small GTPases. To test that hypothesis we have investigated the effects of perillic acid, an agent reported to inhibit isoprenylation, on rat lenses in organ culture. Perillic acid caused dose and time dependent opacification of cultured lenses. While the opacities appeared grossly similar to those produced by lovastatin, they differed dramatically when analysed histologically. It also produced marked morphological changes to lens epithelial cells in culture. Analysis of small GTPases in the perillic acid treated cells failed to detect any accumulation in the water soluble fraction as would be expected if isoprenylation was inhibited. Further, studies on the isoprenylation of radiolabelled isoprenoids into proteins in cultured lenses showed no significant decrease following perillic acid exposure. It was concluded that perillic acid causes cataract in this system by a mechanism different from lovastatin and that inhibition of isoprenylation is unlikely to be a primary factor in the perillic acid cataract.

Duke Scholars

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

August 2001

Volume

73

Issue

2

Start / End Page

239 / 245

Location

England

Related Subject Headings

  • Terpenes
  • Rats, Sprague-Dawley
  • Rats
  • Protein Prenylation
  • Organ Culture Techniques
  • Ophthalmology & Optometry
  • Monoterpenes
  • Male
  • Lovastatin
  • Lens, Crystalline
 

Citation

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ICMJE
MLA
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Cheng, Q. F., Rao, P. V., & Zigler, J. S. (2001). Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens. Exp Eye Res, 73(2), 239–245. https://doi.org/10.1006/exer.2001.1030
Cheng, Q. F., P. V. Rao, and J. S. Zigler. “Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens.Exp Eye Res 73, no. 2 (August 2001): 239–45. https://doi.org/10.1006/exer.2001.1030.
Cheng QF, Rao PV, Zigler JS. Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens. Exp Eye Res. 2001 Aug;73(2):239–45.
Cheng, Q. F., et al. “Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens.Exp Eye Res, vol. 73, no. 2, Aug. 2001, pp. 239–45. Pubmed, doi:10.1006/exer.2001.1030.
Cheng QF, Rao PV, Zigler JS. Effect of perillic acid, a putative isoprenylation inhibitor, on the cultured rat lens. Exp Eye Res. 2001 Aug;73(2):239–245.
Journal cover image

Published In

Exp Eye Res

DOI

ISSN

0014-4835

Publication Date

August 2001

Volume

73

Issue

2

Start / End Page

239 / 245

Location

England

Related Subject Headings

  • Terpenes
  • Rats, Sprague-Dawley
  • Rats
  • Protein Prenylation
  • Organ Culture Techniques
  • Ophthalmology & Optometry
  • Monoterpenes
  • Male
  • Lovastatin
  • Lens, Crystalline