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Assessing transcription factor motif drift from noisy decoy sequences.

Publication ,  Journal Article
Reddy, TE; DeLisi, C; Shakhnovich, BE
Published in: Genome Inform
2005

Genome scale identification of transcription factor binding sites (TFBS) is fundamental to understanding the complexities of mRNA expression at both the cell and organismal levels. While high-throughput experimental methods provide associations between transcription factors and the genes they regulate under a specified experimental condition, computational methods are still required to pinpoint the exact location of binding. Moreover, since the binding site is an intrinsic property of the promoter region, computational methods are in principle more general than condition dependent experimental methods. Computational identification of TFBSs is complicated in at least two different ways. First, transcription factors bind a heterogeneous distribution of sites and therefore have a distribution of affinities. Second, the set of sequences for which a common site is to be determined do not all have a site for the TF of interest. In this paper, we evaluate the robustness of TFBS identification with respect to both effects. We show addition of upstream regions that do not have the TFBS destroy the specificity of the predicted binding site. We also propose a method to calculate the distance between position weight matrices that can be used to measure "drift'' from the canonical binding site. The results presented here could be useful in developing future transcription factor binding site identification algorithms.

Duke Scholars

Published In

Genome Inform

ISSN

0919-9454

Publication Date

2005

Volume

16

Issue

1

Start / End Page

59 / 67

Location

Japan

Related Subject Headings

  • Transcription Factors
  • Saccharomyces cerevisiae
  • Protein Binding
  • Promoter Regions, Genetic
  • Microarray Analysis
  • Genome, Fungal
  • DNA-Binding Proteins
  • DNA
  • Computational Biology
  • Chromatin Immunoprecipitation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Reddy, T. E., DeLisi, C., & Shakhnovich, B. E. (2005). Assessing transcription factor motif drift from noisy decoy sequences. Genome Inform, 16(1), 59–67.
Reddy, Timothy E., Charles DeLisi, and Boris E. Shakhnovich. “Assessing transcription factor motif drift from noisy decoy sequences.Genome Inform 16, no. 1 (2005): 59–67.
Reddy TE, DeLisi C, Shakhnovich BE. Assessing transcription factor motif drift from noisy decoy sequences. Genome Inform. 2005;16(1):59–67.
Reddy, Timothy E., et al. “Assessing transcription factor motif drift from noisy decoy sequences.Genome Inform, vol. 16, no. 1, 2005, pp. 59–67.
Reddy TE, DeLisi C, Shakhnovich BE. Assessing transcription factor motif drift from noisy decoy sequences. Genome Inform. 2005;16(1):59–67.

Published In

Genome Inform

ISSN

0919-9454

Publication Date

2005

Volume

16

Issue

1

Start / End Page

59 / 67

Location

Japan

Related Subject Headings

  • Transcription Factors
  • Saccharomyces cerevisiae
  • Protein Binding
  • Promoter Regions, Genetic
  • Microarray Analysis
  • Genome, Fungal
  • DNA-Binding Proteins
  • DNA
  • Computational Biology
  • Chromatin Immunoprecipitation