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Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires.

Publication ,  Journal Article
Polikov, VS; Hong, J-S; Reichert, WM
Published in: Journal of neuroscience methods
July 2010

A basal lamina gel preparation was incorporated into a modified neuroinflammation cell culture model to test the system as a characterization tool for surface-modified microwires. The extent of gliosis at the surface of gel-coated microwires was quantified in response to titrating the cell culture with a number of soluble factors reported to be involved in reactive gliosis. Positive control conditions (1% FBS, 10 ng/ml bFGF, Neural Basal medium with B27 supplement) induced a layer of GFAP-expressing astrocytes to accumulate on all gel-coated microwires. Serum, inflammatory cytokines IL-1alpha and IL-1beta and the neural progenitor cell (NPC) proliferating growth factors bFGF and PDGF all increased the number of reactive cells on the gel, in agreement with their reported roles in cell activation, migration and proliferation at the site of injury. Technically, this study shows that a fetal neuron-glia culture system is well suited for characterizing the impact of electrode coatings designed to mitigate implant-associated gliosis, and for screening the effect of multiple soluble factors that promote and/or inhibit implant-associated gliosis. Scientifically, this study points to essential roles of serum and inflammatory factors to induce NPC activation and migration to the site of injury, where growth factors like bFGF and PDGF induce proliferation of cells that will eventually form the glial scar.

Duke Scholars

Published In

Journal of neuroscience methods

DOI

EISSN

1872-678X

ISSN

0165-0270

Publication Date

July 2010

Volume

190

Issue

2

Start / End Page

180 / 187

Related Subject Headings

  • Surface Properties
  • Serum
  • Rats, Inbred F344
  • Rats
  • Neurons
  • Neurology & Neurosurgery
  • Neuroglia
  • Microtechnology
  • Microelectrodes
  • Mesencephalon
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Polikov, V. S., Hong, J.-S., & Reichert, W. M. (2010). Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires. Journal of Neuroscience Methods, 190(2), 180–187. https://doi.org/10.1016/j.jneumeth.2010.05.002
Polikov, Vadim S., Jau-Shyong Hong, and William M. Reichert. “Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires.Journal of Neuroscience Methods 190, no. 2 (July 2010): 180–87. https://doi.org/10.1016/j.jneumeth.2010.05.002.
Polikov VS, Hong J-S, Reichert WM. Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires. Journal of neuroscience methods. 2010 Jul;190(2):180–7.
Polikov, Vadim S., et al. “Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires.Journal of Neuroscience Methods, vol. 190, no. 2, July 2010, pp. 180–87. Epmc, doi:10.1016/j.jneumeth.2010.05.002.
Polikov VS, Hong J-S, Reichert WM. Soluble factor effects on glial cell reactivity at the surface of gel-coated microwires. Journal of neuroscience methods. 2010 Jul;190(2):180–187.
Journal cover image

Published In

Journal of neuroscience methods

DOI

EISSN

1872-678X

ISSN

0165-0270

Publication Date

July 2010

Volume

190

Issue

2

Start / End Page

180 / 187

Related Subject Headings

  • Surface Properties
  • Serum
  • Rats, Inbred F344
  • Rats
  • Neurons
  • Neurology & Neurosurgery
  • Neuroglia
  • Microtechnology
  • Microelectrodes
  • Mesencephalon