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Migration assay to measure cellular response to signalling gradients

Publication ,  Journal Article
Smith, JT; Ma, H; Reichert, WM
Published in: Transactions - 7th World Biomaterials Congress
December 1, 2004

The use of a modified Teflon fence assay to track the movement of endothelical cells in response to a diffusion generated signalling gradient of fibronectin was discussed. The substrates were generated from self-assembled monolayers of alkanethoils on optically transparent Au (silver) coated glass slides. Bovine aortic cells (BAEC) were grown to a confluent monolayer behind a Teflon fence positioned at 20% of the maximum fibronectin concentration on the gradients. Cells were cultured in Dulbecccco's modified eagles medium supplemented with 10% serum and 2% penicillin/streptomycin during seeding. It was observed that the gradient and the control substrates had velocity in the X direction over the first 9 hours of the migration assay.

Duke Scholars

Published In

Transactions - 7th World Biomaterials Congress

Publication Date

December 1, 2004

Start / End Page

75
 

Citation

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Smith, J. T., Ma, H., & Reichert, W. M. (2004). Migration assay to measure cellular response to signalling gradients. Transactions - 7th World Biomaterials Congress, 75.
Smith, J. T., H. Ma, and W. M. Reichert. “Migration assay to measure cellular response to signalling gradients.” Transactions - 7th World Biomaterials Congress, December 1, 2004, 75.
Smith JT, Ma H, Reichert WM. Migration assay to measure cellular response to signalling gradients. Transactions - 7th World Biomaterials Congress. 2004 Dec 1;75.
Smith, J. T., et al. “Migration assay to measure cellular response to signalling gradients.” Transactions - 7th World Biomaterials Congress, Dec. 2004, p. 75.
Smith JT, Ma H, Reichert WM. Migration assay to measure cellular response to signalling gradients. Transactions - 7th World Biomaterials Congress. 2004 Dec 1;75.

Published In

Transactions - 7th World Biomaterials Congress

Publication Date

December 1, 2004

Start / End Page

75