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Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model.

Publication ,  Journal Article
Ashley, RA; Roth, CC; Palmer, BW; Kibar, Y; Routh, JC; Fung, K-M; Frimberger, D; Lin, H-K; Kropp, BP
Published in: BJU Int
May 2010

OBJECTIVE: To examine the histological differences in the inflammatory response and regenerative outcomes of distal vs proximal porcine small intestinal submucosa (SIS) grafts in the rat bladder, as SIS from distal small intestine yields reliable and reproducible bladder regeneration, while SIS from proximal portions of small intestine does not provide similar results. MATERIALS AND METHODS: In all, 30 Sprague-Dawley rats underwent hemi-cystectomy followed by anastomosis of a bladder patch of SIS prepared from either distal or proximal small intestine. After bladder harvest, immunohistochemistry was used to quantify mast cells, eosinophils, macrophages, and neutrophils (PMNs). Total cell count per unit area was compared across the time course in univariate and logistic regression modelling. RESULTS: There were more eosinophils and mast cells in proximal SIS grafts, while there were more macrophages and PMNs in distal SIS grafts (all P < 0.05). Trichrome analysis showed increased collagen deposition in proximal SIS grafts and little smooth muscle regeneration. There was also significant graft contracture in proximal SIS grafts compared with distal SIS grafts (P < 0.05). CONCLUSIONS: We conclude that the location of SIS origin may evoke different inflammatory responses, which results in altered bladder tissue regeneration.

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Published In

BJU Int

DOI

EISSN

1464-410X

Publication Date

May 2010

Volume

105

Issue

10

Start / End Page

1462 / 1468

Location

England

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Swine
  • Regeneration
  • Rats, Sprague-Dawley
  • Rats
  • Neutrophils
  • Intestine, Small
  • Intestinal Mucosa
  • Immunohistochemistry
 

Citation

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Chicago
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Ashley, R. A., Roth, C. C., Palmer, B. W., Kibar, Y., Routh, J. C., Fung, K.-M., … Kropp, B. P. (2010). Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model. BJU Int, 105(10), 1462–1468. https://doi.org/10.1111/j.1464-410X.2009.08965.x
Ashley, Richard A., Christopher C. Roth, Blake W. Palmer, Yusuf Kibar, Jonathan C. Routh, Kar-Ming Fung, Dominic Frimberger, Hsueh-Kung Lin, and Bradley P. Kropp. “Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model.BJU Int 105, no. 10 (May 2010): 1462–68. https://doi.org/10.1111/j.1464-410X.2009.08965.x.
Ashley, Richard A., et al. “Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model.BJU Int, vol. 105, no. 10, May 2010, pp. 1462–68. Pubmed, doi:10.1111/j.1464-410X.2009.08965.x.
Ashley RA, Roth CC, Palmer BW, Kibar Y, Routh JC, Fung K-M, Frimberger D, Lin H-K, Kropp BP. Regional variations in small intestinal submucosa evoke differences in inflammation with subsequent impact on tissue regeneration in the rat bladder augmentation model. BJU Int. 2010 May;105(10):1462–1468.
Journal cover image

Published In

BJU Int

DOI

EISSN

1464-410X

Publication Date

May 2010

Volume

105

Issue

10

Start / End Page

1462 / 1468

Location

England

Related Subject Headings

  • Urology & Nephrology
  • Urinary Bladder
  • Swine
  • Regeneration
  • Rats, Sprague-Dawley
  • Rats
  • Neutrophils
  • Intestine, Small
  • Intestinal Mucosa
  • Immunohistochemistry