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An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells.

Publication ,  Journal Article
Dotan, S; Pinkas, A; Slotkin, TA; Yanai, J
Published in: Neurotoxicol Teratol
2010

A fast and simple model which uses lower animals on the evolutionary scale is beneficial for developing procedures for the reversal of neurobehavioral teratogenicity with neural stem cells. Here, we established a procedure for the derivation of chick neural stem cells, establishing embryonic day (E) 10 as optimal for progression to neuronal phenotypes. Cells were obtained from the embryonic cerebral hemispheres and incubated for 5-7 days in enriched medium containing epidermal growth factor (EGF) and basic fibroblast growth factor (FGF2) according to a procedure originally developed for mice. A small percentage of the cells survived, proliferated and formed nestin-positive neurospheres. After removal of the growth factors to allow differentiation (5 days), 74% of the cells differentiated into all major lineages of the nervous system, including neurons (Beta III tubulin-positive, 54% of the total number of differentiated cells), astrocytes (GFAP-positive, 26%), and oligodendrocytes (O4-positive, 20%). These findings demonstrate that the cells were indeed neural stem cells. Next, the cells were transplanted in two allograft chick models; (1) direct cerebral transplantation to 24-h-old chicks, followed by post-transplantation cell tracking at 24 h, 6 days and 14 days, and (2) intravenous transplantation to chick embryos on E13, followed by cell tracking on E19. With both methods, transplanted cells were found in the brain. The chick embryo provides a convenient, precisely-timed and unlimited supply of neural progenitors for therapy by transplantation, as well as constituting a fast and simple model in which to evaluate the ability of neural stem cell transplantation to repair neural damage, steps that are critical for progress toward therapeutic applications.

Duke Scholars

Published In

Neurotoxicol Teratol

DOI

EISSN

1872-9738

Publication Date

2010

Volume

32

Issue

4

Start / End Page

481 / 488

Location

United States

Related Subject Headings

  • Toxicology
  • Toxicity Tests
  • Stem Cell Transplantation
  • Oligodendroglia
  • Neurons
  • Fibroblast Growth Factor 2
  • Epidermal Growth Factor
  • Embryonic Stem Cells
  • Embryo, Nonmammalian
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dotan, S., Pinkas, A., Slotkin, T. A., & Yanai, J. (2010). An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells. Neurotoxicol Teratol, 32(4), 481–488. https://doi.org/10.1016/j.ntt.2010.02.003
Dotan, Sharon, Adi Pinkas, Theodore A. Slotkin, and Joseph Yanai. “An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells.Neurotoxicol Teratol 32, no. 4 (2010): 481–88. https://doi.org/10.1016/j.ntt.2010.02.003.
Dotan S, Pinkas A, Slotkin TA, Yanai J. An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells. Neurotoxicol Teratol. 2010;32(4):481–8.
Dotan, Sharon, et al. “An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells.Neurotoxicol Teratol, vol. 32, no. 4, 2010, pp. 481–88. Pubmed, doi:10.1016/j.ntt.2010.02.003.
Dotan S, Pinkas A, Slotkin TA, Yanai J. An avian model for the reversal of neurobehavioral teratogenicity with neural stem cells. Neurotoxicol Teratol. 2010;32(4):481–488.
Journal cover image

Published In

Neurotoxicol Teratol

DOI

EISSN

1872-9738

Publication Date

2010

Volume

32

Issue

4

Start / End Page

481 / 488

Location

United States

Related Subject Headings

  • Toxicology
  • Toxicity Tests
  • Stem Cell Transplantation
  • Oligodendroglia
  • Neurons
  • Fibroblast Growth Factor 2
  • Epidermal Growth Factor
  • Embryonic Stem Cells
  • Embryo, Nonmammalian
  • Disease Models, Animal