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3H-dopamine uptake by synaptic storage vesicles of rat whole brain and brain regions

Publication ,  Journal Article
Slotkin, TA; Salvaggio, M; Lau, C; Kirksey, DF
Published in: Life Sciences
1978

A crude preparation of neurotransmitter storage vesicles was obtained by differential centrifugation and the ability to take up 3H-dopamine was evaluated in vitro. The uptake was highly dependent on temperature, had an absolute requirement for ATP and Mg2+ and was inhibited totally by reserpine. The uptake displayed saturation kinetics, with a Km of 0.26 μM at 20°. 3H-dopamine uptake was inhibited competitively by norepinephrine, with a Ki of 0.69 μM. Vesicles derived from a primarily dopaminergic region (corpus striatum) exhibited the same ratio of uptakes of 3H-dopamine/3H-norepinephrine as did those from a primarily noradrenergic region (cerebral cortex). These results indicate that viable rat brain storage vesicles can be readily prepared and used for evaluation of pharmacologic effects on 3H-dopamine uptake, and that dopaminergic and noradrenergic storage vesicles exhibit identical uptake properties. © 1978.

Duke Scholars

Published In

Life Sciences

ISSN

0024-3205

Publication Date

1978

Volume

22

Issue

10

Start / End Page

823 / 830

Related Subject Headings

  • Pharmacology & Pharmacy
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0601 Biochemistry and Cell Biology
 

Citation

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Slotkin, T. A., Salvaggio, M., Lau, C., & Kirksey, D. F. (1978). 3H-dopamine uptake by synaptic storage vesicles of rat whole brain and brain regions. Life Sciences, 22(10), 823–830.
Slotkin, T. A., M. Salvaggio, C. Lau, and D. F. Kirksey. “3H-dopamine uptake by synaptic storage vesicles of rat whole brain and brain regions.” Life Sciences 22, no. 10 (1978): 823–30.
Slotkin TA, Salvaggio M, Lau C, Kirksey DF. 3H-dopamine uptake by synaptic storage vesicles of rat whole brain and brain regions. Life Sciences. 1978;22(10):823–30.
Slotkin, T. A., et al. “3H-dopamine uptake by synaptic storage vesicles of rat whole brain and brain regions.” Life Sciences, vol. 22, no. 10, 1978, pp. 823–30.
Slotkin TA, Salvaggio M, Lau C, Kirksey DF. 3H-dopamine uptake by synaptic storage vesicles of rat whole brain and brain regions. Life Sciences. 1978;22(10):823–830.
Journal cover image

Published In

Life Sciences

ISSN

0024-3205

Publication Date

1978

Volume

22

Issue

10

Start / End Page

823 / 830

Related Subject Headings

  • Pharmacology & Pharmacy
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0601 Biochemistry and Cell Biology