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Identification of additional members of human G-protein-coupled receptor kinase multigene family.

Publication ,  Journal Article
Haribabu, B; Snyderman, R
Published in: Proc Natl Acad Sci U S A
October 15, 1993

Human neutrophils express several distinct guanine nucleotide binding (G)-protein-coupled receptors that mediate their responsiveness to chemoattractants. Phosphorylation by receptor-specific and second messenger-activated protein kinases is a common mechanism for regulation of G-protein-coupled receptors. To explore the possibility that chemoattractant receptors are regulated by unique receptor kinases, we utilized PCR to identify receptor kinases in human neutrophils. Here, we report the isolation of three G-protein-coupled-receptor-kinase (GPRK)-like sequences termed GPRK5, GPRK6, and GPRK7 in addition to the beta-adrenergic receptor kinase (beta ARK) 1 and 2 isoforms (beta ARK1 and beta ARK2). Two, GPRK5 and GPRK6, showed high homology at the amino acid level to the recently identified receptor-kinase-like sequence localized close to the Huntington disease locus. GPRK7 is of interest in that it contains a DLG (Asp-Leu-Gly) amino acid motif of receptor kinases preceded by a DFD (Asp-Phe-Asp) motif. We isolated cDNAs corresponding to GPRK6; the complete sequence shows > 66% identity and 81% similarity at the amino acid level to the GPRK from the Huntington disease locus. The GPRK6 cDNA probe hybridizes to two mRNAs of 2.9 and 2.1 kb that were expressed in all the tested human tissues including HL-60 cells and neutrophils. Genomic Southern blot analysis and chromosome mapping showed that GPRK6 hybridizes to two closely related genes located on chromosomes 5 and 13 and are, therefore, distinct from the GPRK located near the Huntington disease locus on chromosome 4. The identification herein of three putative receptor kinases indicates that in addition to beta ARK and rhodopsin kinase subfamilies, there are other receptor-kinase subfamilies that regulate the broad spectrum of G-protein-coupled receptors.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 15, 1993

Volume

90

Issue

20

Start / End Page

9398 / 9402

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Receptors, Cell Surface
  • Protein Kinases
  • Neutrophils
  • Multigene Family
  • Molecular Sequence Data
  • Genes
  • GTP-Binding Proteins
  • DNA, Complementary
 

Citation

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Haribabu, B., & Snyderman, R. (1993). Identification of additional members of human G-protein-coupled receptor kinase multigene family. Proc Natl Acad Sci U S A, 90(20), 9398–9402. https://doi.org/10.1073/pnas.90.20.9398
Haribabu, B., and R. Snyderman. “Identification of additional members of human G-protein-coupled receptor kinase multigene family.Proc Natl Acad Sci U S A 90, no. 20 (October 15, 1993): 9398–9402. https://doi.org/10.1073/pnas.90.20.9398.
Haribabu B, Snyderman R. Identification of additional members of human G-protein-coupled receptor kinase multigene family. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9398–402.
Haribabu, B., and R. Snyderman. “Identification of additional members of human G-protein-coupled receptor kinase multigene family.Proc Natl Acad Sci U S A, vol. 90, no. 20, Oct. 1993, pp. 9398–402. Pubmed, doi:10.1073/pnas.90.20.9398.
Haribabu B, Snyderman R. Identification of additional members of human G-protein-coupled receptor kinase multigene family. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9398–9402.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 15, 1993

Volume

90

Issue

20

Start / End Page

9398 / 9402

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Receptors, Cell Surface
  • Protein Kinases
  • Neutrophils
  • Multigene Family
  • Molecular Sequence Data
  • Genes
  • GTP-Binding Proteins
  • DNA, Complementary