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Amphotericin B alters the affinity and functional activity of the oligopeptide chemotactic factor receptor on human polymorphonuclear leukocytes.

Publication ,  Journal Article
Lohr, KM; Snyderman, R
Published in: J Immunol
October 1982

Leukocyte chemotaxis is initiated by the binding of chemotactic factors to specific, high-affinity receptors. Amphotericin B, a polyene antibiotic that binds to membrane cholesterol, inhibits human neutrophil (PMN) chemotaxis. We examined the effects of this drug on PMN functions mediated by the oligopeptide chemotactic factor receptor. The antibiotic irreversibly inhibited chemotaxis and depressed the binding of the radiolabeled chemoattractant, fMet-Leu-[3H]Phe, to its receptor without affecting the receptor's specificity. The drug lowered the binding affinity of the receptor by up to fivefold and slightly increased its number. Doses of amphotericin B that depressed receptor affinity and inhibited chemotaxis did not diminish lysosomal enzyme secretion or superoxide anion production. Nystatin, a less potent polyene antibiotic, also diminished chemotactic factor binding, but to a lesser degree than amphotericin B did. A chemically unrelated antifungal agent had no effect on either binding or chemotaxis. Thus, pharmacologic manipulation can alter the affinity of the chemotactic factor receptor on human PMN; this alteration is associated with a change in receptor function. The data suggest that receptor affinity regulates or at least reflects its functional state, and that the transduction mechanisms for various biologic responses mediated by the chemoattractant receptor are heterogeneous. By pharmacologic alterations of receptor affinity, one may be able to modulate specific biologic responses elicited by chemoattractant receptor-ligand interactions.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 1982

Volume

129

Issue

4

Start / End Page

1594 / 1599

Location

United States

Related Subject Headings

  • Superoxides
  • Structure-Activity Relationship
  • Receptors, Formyl Peptide
  • Receptors, Cell Surface
  • Oligopeptides
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • N-Formylmethionine
  • Methionine
  • Membrane Lipids
 

Published In

J Immunol

ISSN

0022-1767

Publication Date

October 1982

Volume

129

Issue

4

Start / End Page

1594 / 1599

Location

United States

Related Subject Headings

  • Superoxides
  • Structure-Activity Relationship
  • Receptors, Formyl Peptide
  • Receptors, Cell Surface
  • Oligopeptides
  • Neutrophils
  • N-Formylmethionine Leucyl-Phenylalanine
  • N-Formylmethionine
  • Methionine
  • Membrane Lipids