Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells.
Publication
, Journal Article
Levy, NL; Synderman, R; Ladda, RL; Lieberman, R
Published in: Proc Natl Acad Sci U S A
November 1973
Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5-deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5-Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic "repair" in mammals.
Duke Scholars
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
November 1973
Volume
70
Issue
11
Start / End Page
3125 / 3129
Location
United States
Related Subject Headings
- Transplantation, Homologous
- Transplantation, Heterologous
- Spleen
- Species Specificity
- Parainfluenza Virus 1, Human
- Macrophages
- Kidney
- Immunologic Deficiency Syndromes
- Immunodiffusion
- Immunoassay
Citation
APA
Chicago
ICMJE
MLA
NLM
Levy, N. L., Synderman, R., Ladda, R. L., & Lieberman, R. (1973). Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells. Proc Natl Acad Sci U S A, 70(11), 3125–3129. https://doi.org/10.1073/pnas.70.11.3125
Levy, N. L., R. Synderman, R. L. Ladda, and R. Lieberman. “Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells.” Proc Natl Acad Sci U S A 70, no. 11 (November 1973): 3125–29. https://doi.org/10.1073/pnas.70.11.3125.
Levy NL, Synderman R, Ladda RL, Lieberman R. Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3125–9.
Levy, N. L., et al. “Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells.” Proc Natl Acad Sci U S A, vol. 70, no. 11, Nov. 1973, pp. 3125–29. Pubmed, doi:10.1073/pnas.70.11.3125.
Levy NL, Synderman R, Ladda RL, Lieberman R. Cytogenetic engineering in vivo: restoration of biologic complement activity to C5-deficient mice by intravenous inoculation of hybrid cells. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3125–3129.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
November 1973
Volume
70
Issue
11
Start / End Page
3125 / 3129
Location
United States
Related Subject Headings
- Transplantation, Homologous
- Transplantation, Heterologous
- Spleen
- Species Specificity
- Parainfluenza Virus 1, Human
- Macrophages
- Kidney
- Immunologic Deficiency Syndromes
- Immunodiffusion
- Immunoassay