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The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP.

Publication ,  Journal Article
Cabrero, P; Radford, JC; Broderick, KE; Costes, L; Veenstra, JA; Spana, EP; Davies, SA; Dow, JAT
Published in: The Journal of experimental biology
December 2002

Dh, the gene that encodes a CRF-like peptide in Drosophila melanogaster, is described. The product of this gene is a 44-amino-acid peptide (Drome-DH(44)) with a sequence almost identical to the Musca domestica and Stomoxys calcitrans diuretic hormones. There are no other similar peptides encoded within the known Drosophila genomic sequence. Functional studies showed that the deduced peptide stimulated fluid production, and that this effect was mediated by cyclic AMP in principal cells only: there was no effect on the levels of either cyclic GMP or intracellular calcium. Stimulation also elevated levels of cyclic AMP (but not cyclic GMP) phosphodiesterase, a new mode of action for this class of hormone. The transcript was localised by in situ hybridisation, and the peptide by immunocytochemistry, to two groups of three neurones in the pars intercerebralis within the brain. These cells also express receptors for leucokinin, another major diuretic peptide, implying that the cells may be important in homeostatic regulation.

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Published In

The Journal of experimental biology

DOI

EISSN

1477-9145

ISSN

0022-0949

Publication Date

December 2002

Volume

205

Issue

Pt 24

Start / End Page

3799 / 3807

Related Subject Headings

  • Sequence Alignment
  • Receptors, Neuropeptide
  • Physiology
  • Phosphoric Diester Hydrolases
  • Peptides
  • Molecular Sequence Data
  • Larva
  • Insect Hormones
  • Drosophila melanogaster
  • Drosophila Proteins
 

Citation

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Cabrero, P., Radford, J. C., Broderick, K. E., Costes, L., Veenstra, J. A., Spana, E. P., … Dow, J. A. T. (2002). The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP. The Journal of Experimental Biology, 205(Pt 24), 3799–3807. https://doi.org/10.1242/jeb.205.24.3799
Cabrero, Pablo, Jonathan C. Radford, Kate E. Broderick, Laurence Costes, Jan A. Veenstra, Eric P. Spana, Shireen A. Davies, and Julian A. T. Dow. “The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP.The Journal of Experimental Biology 205, no. Pt 24 (December 2002): 3799–3807. https://doi.org/10.1242/jeb.205.24.3799.
Cabrero P, Radford JC, Broderick KE, Costes L, Veenstra JA, Spana EP, et al. The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP. The Journal of experimental biology. 2002 Dec;205(Pt 24):3799–807.
Cabrero, Pablo, et al. “The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP.The Journal of Experimental Biology, vol. 205, no. Pt 24, Dec. 2002, pp. 3799–807. Epmc, doi:10.1242/jeb.205.24.3799.
Cabrero P, Radford JC, Broderick KE, Costes L, Veenstra JA, Spana EP, Davies SA, Dow JAT. The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP. The Journal of experimental biology. 2002 Dec;205(Pt 24):3799–3807.
Journal cover image

Published In

The Journal of experimental biology

DOI

EISSN

1477-9145

ISSN

0022-0949

Publication Date

December 2002

Volume

205

Issue

Pt 24

Start / End Page

3799 / 3807

Related Subject Headings

  • Sequence Alignment
  • Receptors, Neuropeptide
  • Physiology
  • Phosphoric Diester Hydrolases
  • Peptides
  • Molecular Sequence Data
  • Larva
  • Insect Hormones
  • Drosophila melanogaster
  • Drosophila Proteins