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Aptamer-mediated delivery of chemotherapy to pancreatic cancer cells.

Publication ,  Journal Article
Ray, P; Cheek, MA; Sharaf, ML; Li, N; Ellington, AD; Sullenger, BA; Shaw, BR; White, RR
Published in: Nucleic Acid Ther
October 2012

Gemcitabine is a nucleoside analog that is currently the best available single-agent chemotherapeutic drug for pancreatic cancer. However, efficacy is limited by our inability to deliver sufficient active metabolite into cancer cells without toxic effects on normal tissues. Targeted delivery of gemcitabine into cancer cells could maximize effectiveness and concurrently minimize toxic side effects by reducing uptake into normal cells. Most pancreatic cancers overexpress epidermal growth factor receptor (EGFR), a trans-membrane receptor tyrosine kinase. We utilized a nuclease resistant RNA aptamer that binds and is internalized by EGFR on pancreatic cancer cells to deliver gemcitabine-containing polymers into EGFR-expressing cells and inhibit cell proliferation in vitro. This approach to cell type-specific therapy can be adapted to other targets and to other types of therapeutic cargo.

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Published In

Nucleic Acid Ther

DOI

EISSN

2159-3345

Publication Date

October 2012

Volume

22

Issue

5

Start / End Page

295 / 305

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Pancreatic Neoplasms
  • Molecular Sequence Data
  • Microscopy, Fluorescence
  • Humans
  • Gemcitabine
  • Fluorescent Dyes
  • ErbB Receptors
  • Endocytosis
  • Drug Screening Assays, Antitumor
 

Citation

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Ray, P., Cheek, M. A., Sharaf, M. L., Li, N., Ellington, A. D., Sullenger, B. A., … White, R. R. (2012). Aptamer-mediated delivery of chemotherapy to pancreatic cancer cells. Nucleic Acid Ther, 22(5), 295–305. https://doi.org/10.1089/nat.2012.0353
Ray, Partha, Marcus A. Cheek, Mariam L. Sharaf, Na Li, Andrew D. Ellington, Bruce A. Sullenger, Barbara Ramsay Shaw, and Rebekah R. White. “Aptamer-mediated delivery of chemotherapy to pancreatic cancer cells.Nucleic Acid Ther 22, no. 5 (October 2012): 295–305. https://doi.org/10.1089/nat.2012.0353.
Ray P, Cheek MA, Sharaf ML, Li N, Ellington AD, Sullenger BA, et al. Aptamer-mediated delivery of chemotherapy to pancreatic cancer cells. Nucleic Acid Ther. 2012 Oct;22(5):295–305.
Ray, Partha, et al. “Aptamer-mediated delivery of chemotherapy to pancreatic cancer cells.Nucleic Acid Ther, vol. 22, no. 5, Oct. 2012, pp. 295–305. Pubmed, doi:10.1089/nat.2012.0353.
Ray P, Cheek MA, Sharaf ML, Li N, Ellington AD, Sullenger BA, Shaw BR, White RR. Aptamer-mediated delivery of chemotherapy to pancreatic cancer cells. Nucleic Acid Ther. 2012 Oct;22(5):295–305.
Journal cover image

Published In

Nucleic Acid Ther

DOI

EISSN

2159-3345

Publication Date

October 2012

Volume

22

Issue

5

Start / End Page

295 / 305

Location

United States

Related Subject Headings

  • Staining and Labeling
  • Pancreatic Neoplasms
  • Molecular Sequence Data
  • Microscopy, Fluorescence
  • Humans
  • Gemcitabine
  • Fluorescent Dyes
  • ErbB Receptors
  • Endocytosis
  • Drug Screening Assays, Antitumor