Skip to main content

CD10/NEP in non-small cell lung carcinomas. Relationship to cellular proliferation.

Publication ,  Journal Article
Ganju, RK; Sunday, M; Tsarwhas, DG; Card, A; Shipp, MA
Published in: J Clin Invest
November 1994

The cell surface metalloproteinase CD10/neutral endopeptidase 24.11 (NEP) hydrolyzes a variety of peptide substrates and reduces cellular responses to specific peptide hormones. Because CD10/NEP modulates peptide-mediated proliferation of small cell carcinomas of the lung (SCLC) and normal fetal bronchial epithelium, we evaluated the enzyme's expression in non-small cell lung carcinomas (NSCLC). Bronchoalveolar and large cell carcinoma cell lines had low levels of CD10/NEP expression whereas squamous, adenosquamous, and adenocarcinoma cell lines had higher and more variable levels of the cell surface enzyme. Regional variations in CD10/NEP immunostaining in primary NSCLC specimens prompted us to correlate CD10/NEP expression with cell growth. In primary carcinomas of the lung, clonal NSCLC cell lines and SV40-transformed fetal airway epithelium, subsets of cells expressed primarily CD10/NEP or the proliferating cell nuclear antigen (PCNA). Cultured airway epithelial cells had the lowest levels of CD10/NEP expression when the highest percentage of cells were actively dividing; in addition, these cells grew more rapidly when cell surface CD10/NEP was inhibited. NSCLC cell lines had receptors for a variety of mitogenic peptides known to be CD10/NEP substrates, underscoring the functional significance of growth-related variability in CD10/NEP expression.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

November 1994

Volume

94

Issue

5

Start / End Page

1784 / 1791

Location

United States

Related Subject Headings

  • Vasoactive Intestinal Peptide
  • Tumor Cells, Cultured
  • Receptors, Bombesin
  • Neprilysin
  • Molecular Sequence Data
  • Lung Neoplasms
  • Immunology
  • Humans
  • Cell Division
  • Carcinoma, Non-Small-Cell Lung
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ganju, R. K., Sunday, M., Tsarwhas, D. G., Card, A., & Shipp, M. A. (1994). CD10/NEP in non-small cell lung carcinomas. Relationship to cellular proliferation. J Clin Invest, 94(5), 1784–1791. https://doi.org/10.1172/JCI117526
Ganju, R. K., M. Sunday, D. G. Tsarwhas, A. Card, and M. A. Shipp. “CD10/NEP in non-small cell lung carcinomas. Relationship to cellular proliferation.J Clin Invest 94, no. 5 (November 1994): 1784–91. https://doi.org/10.1172/JCI117526.
Ganju RK, Sunday M, Tsarwhas DG, Card A, Shipp MA. CD10/NEP in non-small cell lung carcinomas. Relationship to cellular proliferation. J Clin Invest. 1994 Nov;94(5):1784–91.
Ganju, R. K., et al. “CD10/NEP in non-small cell lung carcinomas. Relationship to cellular proliferation.J Clin Invest, vol. 94, no. 5, Nov. 1994, pp. 1784–91. Pubmed, doi:10.1172/JCI117526.
Ganju RK, Sunday M, Tsarwhas DG, Card A, Shipp MA. CD10/NEP in non-small cell lung carcinomas. Relationship to cellular proliferation. J Clin Invest. 1994 Nov;94(5):1784–1791.

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

November 1994

Volume

94

Issue

5

Start / End Page

1784 / 1791

Location

United States

Related Subject Headings

  • Vasoactive Intestinal Peptide
  • Tumor Cells, Cultured
  • Receptors, Bombesin
  • Neprilysin
  • Molecular Sequence Data
  • Lung Neoplasms
  • Immunology
  • Humans
  • Cell Division
  • Carcinoma, Non-Small-Cell Lung