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Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications.

Publication ,  Journal Article
Kasili, PM; Vo-Dinh, T
Published in: Cytometry. Part A : the journal of the International Society for Analytical Cytology
August 2006

A major advantage of flow cytometry is its flexible and open instrument configuration, which is highly suitable for systems integration. This flexibility permits the coupling of auxiliary instrumentation that may offer the measurement of parameters other than those typically measured by this multiparameter measurement technique. On the basis of this advantage, we explore the principle and application of hyperspectral imaging (HSI), which has the potential to be a useful add-on feature to flow cytometry applications. Application of HSI to flow cytometry involves the acquisition of spatial information and rendering it in spectral form. In this work, we describe the development and application of an HSI system which provides both spectral and spatial information. Spectral information was generated by obtaining an entire spectrum of a single sample site within a wavelength region of interest, while spatial information was generated by recording a two-dimensional (2D) image of an area of the sample of interest at one specific wavelength. HSI is a promising additional feature to flow cytometry since it can provide both spatial (image format) and spectral information in addition to the multiparameter information already available from flow cytometry measurements.

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Published In

Cytometry. Part A : the journal of the International Society for Analytical Cytology

DOI

EISSN

1552-4930

ISSN

1552-4922

Publication Date

August 2006

Volume

69

Issue

8

Start / End Page

835 / 841

Related Subject Headings

  • Spectrometry, Fluorescence
  • Rhodamine 123
  • Optics and Photonics
  • Immunology
  • Humans
  • Fluorescent Dyes
  • Fluorescein
  • Flow Cytometry
  • Electromagnetic Phenomena
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Kasili, P. M., & Vo-Dinh, T. (2006). Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications. Cytometry. Part A : The Journal of the International Society for Analytical Cytology, 69(8), 835–841. https://doi.org/10.1002/cyto.a.20307
Kasili, Paul M., and Tuan Vo-Dinh. “Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications.Cytometry. Part A : The Journal of the International Society for Analytical Cytology 69, no. 8 (August 2006): 835–41. https://doi.org/10.1002/cyto.a.20307.
Kasili PM, Vo-Dinh T. Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications. Cytometry Part A : the journal of the International Society for Analytical Cytology. 2006 Aug;69(8):835–41.
Kasili, Paul M., and Tuan Vo-Dinh. “Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications.Cytometry. Part A : The Journal of the International Society for Analytical Cytology, vol. 69, no. 8, Aug. 2006, pp. 835–41. Epmc, doi:10.1002/cyto.a.20307.
Kasili PM, Vo-Dinh T. Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications. Cytometry Part A : the journal of the International Society for Analytical Cytology. 2006 Aug;69(8):835–841.
Journal cover image

Published In

Cytometry. Part A : the journal of the International Society for Analytical Cytology

DOI

EISSN

1552-4930

ISSN

1552-4922

Publication Date

August 2006

Volume

69

Issue

8

Start / End Page

835 / 841

Related Subject Headings

  • Spectrometry, Fluorescence
  • Rhodamine 123
  • Optics and Photonics
  • Immunology
  • Humans
  • Fluorescent Dyes
  • Fluorescein
  • Flow Cytometry
  • Electromagnetic Phenomena
  • Animals