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Correlation between interferon- gamma secretion and cytotoxicity, in virus-specific memory T cells.

Publication ,  Journal Article
Horton, H; Russell, N; Moore, E; Frank, I; Baydo, R; Havenar-Daughton, C; Lee, D; Deers, M; Hudgens, M; Weinhold, K; McElrath, MJ
Published in: J Infect Dis
November 1, 2004

ELISpot and intracellular cytokine staining are replacing the traditional cytolytic ((51)Cr release) assay method in vaccine trials using human immunodeficiency virus (HIV)-1, and it is widely assumed that the number of interferon (IFN)- gamma -secreting T cells is a surrogate for the level of cytolytic activity. Thus, we sought to determine whether the detection of IFN- gamma in CD8(+) T cells correlates with cytolytic ability in vitro. In 29 (69.0%) of 42 HIV-1-seronegative immunocompetent individuals (22 unvaccinated and 20 vaccinated), virus-specific T cell responses recognizing cytomegalovirus, Epstein-Barr virus, and influenza and HIV-1 Gag epitopes were detected by at least 1 assay method (ELISpot, intracellular cytokine staining, and/or (51)Cr release), and 18 (62.1%) of these 29 demonstrated both IFN- gamma secretion and cytolysis. There was strong correlation between the results of IFN- gamma ELISpot and those of IFN- gamma intracellular cytokine staining ( rho =0.88) and between the results of (51)Cr release and those of intracellular cytokine staining ( rho =0.81); although the correlation is not absolute, intracellular cytokine staining can be used--and is superior to ELISpot--as a surrogate for cytolytic assays.

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Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

November 1, 2004

Volume

190

Issue

9

Start / End Page

1692 / 1696

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Staining and Labeling
  • Orthomyxoviridae
  • Microbiology
  • Interferon-gamma
  • Immunologic Memory
  • Humans
  • Herpesvirus 4, Human
  • HIV
  • Cytotoxicity, Immunologic
 

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Horton, H., Russell, N., Moore, E., Frank, I., Baydo, R., Havenar-Daughton, C., … McElrath, M. J. (2004). Correlation between interferon- gamma secretion and cytotoxicity, in virus-specific memory T cells. J Infect Dis, 190(9), 1692–1696. https://doi.org/10.1086/424490
Horton, Helen, Nina Russell, Erin Moore, Ian Frank, Ruth Baydo, Colin Havenar-Daughton, Deborah Lee, et al. “Correlation between interferon- gamma secretion and cytotoxicity, in virus-specific memory T cells.J Infect Dis 190, no. 9 (November 1, 2004): 1692–96. https://doi.org/10.1086/424490.
Horton H, Russell N, Moore E, Frank I, Baydo R, Havenar-Daughton C, et al. Correlation between interferon- gamma secretion and cytotoxicity, in virus-specific memory T cells. J Infect Dis. 2004 Nov 1;190(9):1692–6.
Horton, Helen, et al. “Correlation between interferon- gamma secretion and cytotoxicity, in virus-specific memory T cells.J Infect Dis, vol. 190, no. 9, Nov. 2004, pp. 1692–96. Pubmed, doi:10.1086/424490.
Horton H, Russell N, Moore E, Frank I, Baydo R, Havenar-Daughton C, Lee D, Deers M, Hudgens M, Weinhold K, McElrath MJ. Correlation between interferon- gamma secretion and cytotoxicity, in virus-specific memory T cells. J Infect Dis. 2004 Nov 1;190(9):1692–1696.
Journal cover image

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

November 1, 2004

Volume

190

Issue

9

Start / End Page

1692 / 1696

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • Staining and Labeling
  • Orthomyxoviridae
  • Microbiology
  • Interferon-gamma
  • Immunologic Memory
  • Humans
  • Herpesvirus 4, Human
  • HIV
  • Cytotoxicity, Immunologic