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Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain.

Publication ,  Journal Article
Scott, CF; Silver, S; Profy, AT; Putney, SD; Langlois, A; Weinhold, K; Robinson, JE
Published in: Proc Natl Acad Sci U S A
November 1990

We describe a human IgG1 monoclonal antibody (N701.9b) derived by Epstein-Barr virus transformation of B cells from a human immunodeficiency virus-seropositive asymptomatic donor. This antibody was shown to recognize the principal neutralizing domain contained within the V3 region of gp120 of the MN strain of human immunodeficiency virus and MN-like strains, as determined by binding to the PB-1 fragment of MN gp120 and to synthetic peptides corresponding to the V3 region of MN and related virus strains. The epitope identified by monoclonal antibody N701.9b was mapped to a segment of V3 containing at least 7 amino acids (amino acids 316-322), which is located in the "tip" and "right" side of the V3 loop of the MN strain. Furthermore, this antibody manifested potent type-specific fusion-inhibitory activity against the MN strain but not against the IIIB or RF virus strains. This antibody also neutralized four virus isolates that had MN-like V3 region sequences and failed to neutralize three other strains containing unrelated V3 region sequences. Our findings confirm that the V3 region stimulates type-specific neutralizing antibody during natural human immunodeficiency virus infection in humans. The potential clinical use of this antibody is discussed.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 1990

Volume

87

Issue

21

Start / End Page

8597 / 8601

Location

United States

Related Subject Headings

  • Vaccinia virus
  • Species Specificity
  • Neutralization Tests
  • Molecular Sequence Data
  • Humans
  • HIV-1
  • HIV Envelope Protein gp120
  • HIV
  • Epitopes
  • B-Lymphocytes
 

Citation

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Scott, C. F., Silver, S., Profy, A. T., Putney, S. D., Langlois, A., Weinhold, K., & Robinson, J. E. (1990). Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain. Proc Natl Acad Sci U S A, 87(21), 8597–8601. https://doi.org/10.1073/pnas.87.21.8597
Scott, C. F., S. Silver, A. T. Profy, S. D. Putney, A. Langlois, K. Weinhold, and J. E. Robinson. “Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain.Proc Natl Acad Sci U S A 87, no. 21 (November 1990): 8597–8601. https://doi.org/10.1073/pnas.87.21.8597.
Scott CF, Silver S, Profy AT, Putney SD, Langlois A, Weinhold K, et al. Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8597–601.
Scott, C. F., et al. “Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain.Proc Natl Acad Sci U S A, vol. 87, no. 21, Nov. 1990, pp. 8597–601. Pubmed, doi:10.1073/pnas.87.21.8597.
Scott CF, Silver S, Profy AT, Putney SD, Langlois A, Weinhold K, Robinson JE. Human monoclonal antibody that recognizes the V3 region of human immunodeficiency virus gp120 and neutralizes the human T-lymphotropic virus type IIIMN strain. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8597–8601.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 1990

Volume

87

Issue

21

Start / End Page

8597 / 8601

Location

United States

Related Subject Headings

  • Vaccinia virus
  • Species Specificity
  • Neutralization Tests
  • Molecular Sequence Data
  • Humans
  • HIV-1
  • HIV Envelope Protein gp120
  • HIV
  • Epitopes
  • B-Lymphocytes