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Albumin influences sulfobromophthalein transport by hepatocytes of each acinar zone

Publication ,  Journal Article
Gumucio, DL; Gumucio, JJ; Wilson, JAP
Published in: American Journal of Physiology - Gastrointestinal and Liver Physiology
January 1, 1984

To determine whether profiles of decreasing concentration were generated among hepatocytes of the liver acinus during the transport of sulfobromophthalein sodium (BSP), rat livers were perfused with various concentrations of this dye (10 μM to 1 mM) in the presence and absence of albumin. After steady-state conditions for the biliary secretion of BSP had been attained, pieces of liver were rapidly frozen. Following the alkalinization of cryostat-cut sections, the relative concentration of BSP in hepatocytes of each zone and the effect of albumin on this localization were quantitated by microspectrophotometry. The results showed that BSP, perfused in the absence of albumin, was efficiently extracted by the liver (95% on a single pass), generating distinct profiles of decreasing cellular concentration from zone 1 to zone 3 at every concentration of BSP. However, the addition of albumin to the perfusate greatly reduced the extraction of BSP from the sinusoidal compartment and resulted in the abolition of the differences in BSP content between hepatocytes of zone 1 and zone 3. These results represent a direct demonstration that, as predicted by mathematical modeling, binding of BSP to albumin indeed results in a more homogeneous distribution of BSP within the liver acinus. A simple and direct microspectrophotometric method is therefore available to follow the changes in the relative concentration of BSP among the hepatocytes of the various acinar zones.

Duke Scholars

Published In

American Journal of Physiology - Gastrointestinal and Liver Physiology

ISSN

0193-1857

Publication Date

January 1, 1984

Volume

9

Issue

1

Related Subject Headings

  • Gastroenterology & Hepatology
  • 1116 Medical Physiology
  • 0606 Physiology
 

Citation

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MLA
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Gumucio, D. L., Gumucio, J. J., & Wilson, J. A. P. (1984). Albumin influences sulfobromophthalein transport by hepatocytes of each acinar zone. American Journal of Physiology - Gastrointestinal and Liver Physiology, 9(1).
Gumucio, D. L., J. J. Gumucio, and J. A. P. Wilson. “Albumin influences sulfobromophthalein transport by hepatocytes of each acinar zone.” American Journal of Physiology - Gastrointestinal and Liver Physiology 9, no. 1 (January 1, 1984).
Gumucio DL, Gumucio JJ, Wilson JAP. Albumin influences sulfobromophthalein transport by hepatocytes of each acinar zone. American Journal of Physiology - Gastrointestinal and Liver Physiology. 1984 Jan 1;9(1).
Gumucio, D. L., et al. “Albumin influences sulfobromophthalein transport by hepatocytes of each acinar zone.” American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 9, no. 1, Jan. 1984.
Gumucio DL, Gumucio JJ, Wilson JAP. Albumin influences sulfobromophthalein transport by hepatocytes of each acinar zone. American Journal of Physiology - Gastrointestinal and Liver Physiology. 1984 Jan 1;9(1).

Published In

American Journal of Physiology - Gastrointestinal and Liver Physiology

ISSN

0193-1857

Publication Date

January 1, 1984

Volume

9

Issue

1

Related Subject Headings

  • Gastroenterology & Hepatology
  • 1116 Medical Physiology
  • 0606 Physiology