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The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome.

Publication ,  Journal Article
Baumann, CT; Ma, H; Wolford, R; Reyes, JC; Maruvada, P; Lim, C; Yen, PM; Stallcup, MR; Hager, GL
Published in: Mol Endocrinol
April 2001

The glucocorticoid receptor interacting protein-1 (GRIP1) is a member of the steroid receptor coactivator (SRC) family of transcriptional regulators. Green fluorescent protein (GFP) fusions were made to full-length GRIP1, and a series of GRIP1 mutants lacking the defined regulatory regions and the intracellular distribution of these proteins was studied in HeLa cells. The distribution of GRIP1 was complex, ranging from diffuse nucleoplasmic to discrete intranuclear foci. Formation of these foci was dependent on the C-terminal region of GRIP1, which contains the two characterized transcriptional activation domains, AD1 and AD2. A subpopulation of GRIP1 foci associate with ND10s, small nuclear bodies that contain several proteins including PML, SP100, DAXX, and CREB-binding protein (CBP). Association with the ND10s is dependent on the AD1 of GRIP1, a region of the protein previously described as a CBP-interacting domain. The GRIP1 foci are enriched in components of the 26S proteasome, including the core 20S proteasome, PA28alpha, and ubiquitin. In addition, the irreversible proteasome inhibitor lactacystin induced an increase in the total fluorescence intensity of the GFP-GRIP1 expressing cells, demonstrating that GRIP1 is degraded by the proteasome. These findings suggest the intriguing possibility that degradation of GRIP1 by the 26S proteasome may be a key component of its regulation.

Duke Scholars

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

April 2001

Volume

15

Issue

4

Start / End Page

485 / 500

Location

United States

Related Subject Headings

  • Ubiquitins
  • Tumor Suppressor Proteins
  • Transcription Factors
  • Trans-Activators
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • Proteasome Endopeptidase Complex
  • Protease Inhibitors
  • Promyelocytic Leukemia Protein
  • Peptide Hydrolases
 

Citation

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MLA
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Baumann, C. T., Ma, H., Wolford, R., Reyes, J. C., Maruvada, P., Lim, C., … Hager, G. L. (2001). The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome. Mol Endocrinol, 15(4), 485–500. https://doi.org/10.1210/mend.15.4.0618
Baumann, C. T., H. Ma, R. Wolford, J. C. Reyes, P. Maruvada, C. Lim, P. M. Yen, M. R. Stallcup, and G. L. Hager. “The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome.Mol Endocrinol 15, no. 4 (April 2001): 485–500. https://doi.org/10.1210/mend.15.4.0618.
Baumann, C. T., et al. “The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome.Mol Endocrinol, vol. 15, no. 4, Apr. 2001, pp. 485–500. Pubmed, doi:10.1210/mend.15.4.0618.
Baumann CT, Ma H, Wolford R, Reyes JC, Maruvada P, Lim C, Yen PM, Stallcup MR, Hager GL. The glucocorticoid receptor interacting protein 1 (GRIP1) localizes in discrete nuclear foci that associate with ND10 bodies and are enriched in components of the 26S proteasome. Mol Endocrinol. 2001 Apr;15(4):485–500.

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

April 2001

Volume

15

Issue

4

Start / End Page

485 / 500

Location

United States

Related Subject Headings

  • Ubiquitins
  • Tumor Suppressor Proteins
  • Transcription Factors
  • Trans-Activators
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • Proteasome Endopeptidase Complex
  • Protease Inhibitors
  • Promyelocytic Leukemia Protein
  • Peptide Hydrolases