Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Vitamin D receptors repress basal transcription and exert dominant negative activity on triiodothyronine-mediated transcriptional activity.

Publication ,  Journal Article
Yen, PM; Liu, Y; Sugawara, A; Chin, WW
Published in: J Biol Chem
May 3, 1996

We have examined vitamin D receptor (VDR), thyroid hormone receptor (TR), and retinoid X receptor beta (RXR beta) binding to vitamin D response elements (VDREs), two thyroid hormone response elements (TREs) (DR4 and F2), and a retinoic acid response element (DR5). VDR/RXR bound well to the VDREs and to DR4 and DR5 using the electrophoretic mobility shift assay. Surprisingly, VDR/RXR also bound well to F2, which contains half-sites arranged as an inverted palindrome. In co-transfection experiments using CV-1 cells, we observed that VDR repressed basal transcription in the absence of ligand on DR3 and osteopontin VDREs and F2, but had no effect on DR4 or DR5. VDR selectively mediated ligand-dependent transcription on only VDREs. VDR also exhibited dominant negative activity as it blocked triiodothyronine (T3)-mediated transcriptional activity on DR4 and F2. These results demonstrate that VDR/RXR heterodimers can bind promiscuously to a wide range of hormone response elements, including inverted palindromes. Moreover, they show that unliganded VDRs, similar to TRs and retinoic acid receptors, can repress basal transcription. Last, they also suggest a novel repressor function of VDR on T3-mediated transcription which may be significant in tissues where VDR and TR are co-expressed.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 3, 1996

Volume

271

Issue

18

Start / End Page

10910 / 10916

Location

United States

Related Subject Headings

  • Triiodothyronine
  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Retinoid X Receptors
  • Repressor Proteins
  • Recombinant Fusion Proteins
  • Receptors, Retinoic Acid
  • Receptors, Calcitriol
  • Oligodeoxyribonucleotides
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yen, P. M., Liu, Y., Sugawara, A., & Chin, W. W. (1996). Vitamin D receptors repress basal transcription and exert dominant negative activity on triiodothyronine-mediated transcriptional activity. J Biol Chem, 271(18), 10910–10916. https://doi.org/10.1074/jbc.271.18.10910
Yen, P. M., Y. Liu, A. Sugawara, and W. W. Chin. “Vitamin D receptors repress basal transcription and exert dominant negative activity on triiodothyronine-mediated transcriptional activity.J Biol Chem 271, no. 18 (May 3, 1996): 10910–16. https://doi.org/10.1074/jbc.271.18.10910.
Yen, P. M., et al. “Vitamin D receptors repress basal transcription and exert dominant negative activity on triiodothyronine-mediated transcriptional activity.J Biol Chem, vol. 271, no. 18, May 1996, pp. 10910–16. Pubmed, doi:10.1074/jbc.271.18.10910.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

May 3, 1996

Volume

271

Issue

18

Start / End Page

10910 / 10916

Location

United States

Related Subject Headings

  • Triiodothyronine
  • Transcription, Genetic
  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Retinoid X Receptors
  • Repressor Proteins
  • Recombinant Fusion Proteins
  • Receptors, Retinoic Acid
  • Receptors, Calcitriol
  • Oligodeoxyribonucleotides