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Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells.

Publication ,  Journal Article
Liu, Y; Zhang, W
Published in: J Leukoc Biol
September 2008

Transmembrane adaptor proteins couple antigen receptor engagement to downstream signaling cascades in lymphocytes. One example of these proteins is the linker for activation of T cells (LAT), which plays an indispensable role in T cell activation and development. Here, we report identification of a new transmembrane adaptor molecule, namely growth factor receptor-bound protein 2 (Grb2)-binding adaptor protein, transmembrane (GAPT), which is expressed in B cells and myeloid cells. Similar to LAT, GAPT has an extracellular domain, a transmembrane domain, and a cytoplasmic tail with multiple Grb2-binding motifs. In contrast to other transmembrane adaptor proteins, GAPT is not phosphorylated upon BCR ligation but associates with Grb2 constitutively through its proline-rich region. Targeted disruption of the gapt gene in mice affects neither B cell development nor a nitrophenylacetyl-specific antibody response. However, in the absence of GAPT, B cell proliferation after BCR cross-linking is enhanced. In aged GAPT(-/-) mice, the number of marginal zone (MZ) B cells is increased, and other B cell subsets are normal. The serum concentrations of IgM, IgG2b, and IgG3 are also elevated in these mice. These data indicate that GAPT might play an important role in control of B cell activation and proper maintenance of MZ B cells.

Duke Scholars

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Published In

J Leukoc Biol

DOI

ISSN

0741-5400

Publication Date

September 2008

Volume

84

Issue

3

Start / End Page

842 / 851

Location

England

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Retroviridae
  • Receptors, Antigen, B-Cell
  • Rabbits
  • Phosphorylation
  • Molecular Sequence Data
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins
 

Citation

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Liu, Y., & Zhang, W. (2008). Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells. J Leukoc Biol, 84(3), 842–851. https://doi.org/10.1189/jlb.0208087
Liu, Yan, and Weiguo Zhang. “Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells.J Leukoc Biol 84, no. 3 (September 2008): 842–51. https://doi.org/10.1189/jlb.0208087.
Liu, Yan, and Weiguo Zhang. “Identification of a new transmembrane adaptor protein that constitutively binds Grb2 in B cells.J Leukoc Biol, vol. 84, no. 3, Sept. 2008, pp. 842–51. Pubmed, doi:10.1189/jlb.0208087.

Published In

J Leukoc Biol

DOI

ISSN

0741-5400

Publication Date

September 2008

Volume

84

Issue

3

Start / End Page

842 / 851

Location

England

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Retroviridae
  • Receptors, Antigen, B-Cell
  • Rabbits
  • Phosphorylation
  • Molecular Sequence Data
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins