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Transfer of cryopreserved-thawed pre-embryos in a cycle using exogenous steroids without prior gonadotrophin-releasing hormone agonist suppression yields favourable pregnancy results.

Publication ,  Journal Article
Queenan, JT; Ramey, JW; Seltman, HJ; Eure, L; Veeck, LL; Muasher, SJ
Published in: Hum Reprod
June 1997

We have analysed the use of a programmed cycle of administration of exogenous steroids without prior suppression with a gonadotrophin-releasing hormone agonist (GnRHa) for the transfer of cryopreserved-thawed pre-embryos. From July 1992 to June 1994, 199 cycles (162 patients) were studied. Pre-embryos had been previously cryopreserved at the pronuclear stage using 1.5 M 1,2-propanediol as a cryoprotectant. Preparation of the endometrium was achieved in a step-up regime with transdermal oestradiol patches (0.1 to 0.4 mg). Progesterone in oil (50 mg i.m.) was started on cycle day 13. Pre-embryos were thawed on day 14 and transferred on day 15 after evidence of survival and cleavage. The mean (+/- SD) age of patients undergoing transfer was 35.4 +/- 4.3 years. The mean number of pre-embryos thawed was 4.7 +/- 1.8 with a mean of 3.3 +/- 1.4 pre-embryos being transferred. Eight of the cycles demonstrated follicular development >16 mm prior to thaw and transfer; however, these patients did not demonstrate a luteinizing hormone surge. Mean endometrial thickness on day 13 was 10.8 +/- 2.1 mm. Overall pregnancy rate was 29.2% (57/195). The ongoing or delivery rate was 16.1% (32/195). The rate of preclinical losses per transfer was 6.2% (12/195). Overall, the implantation rate was 6.2% (47/757). Thus, the use of a programmed cycle for cryopreserved embryo transfer yields favourable pregnancy outcome and offers practical advantages to patients. Prior suppression with a GnRHa is not necessary for endometrial preparation.

Duke Scholars

Published In

Hum Reprod

DOI

ISSN

0268-1161

Publication Date

June 1997

Volume

12

Issue

6

Start / End Page

1176 / 1180

Location

England

Related Subject Headings

  • Time Factors
  • Retrospective Studies
  • Progesterone
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Menstrual Cycle
  • Infertility
  • Humans
  • Gonadotropin-Releasing Hormone
  • Female
 

Citation

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Queenan, J. T., Ramey, J. W., Seltman, H. J., Eure, L., Veeck, L. L., & Muasher, S. J. (1997). Transfer of cryopreserved-thawed pre-embryos in a cycle using exogenous steroids without prior gonadotrophin-releasing hormone agonist suppression yields favourable pregnancy results. Hum Reprod, 12(6), 1176–1180. https://doi.org/10.1093/humrep/12.6.1176
Queenan, J. T., J. W. Ramey, H. J. Seltman, L. Eure, L. L. Veeck, and S. J. Muasher. “Transfer of cryopreserved-thawed pre-embryos in a cycle using exogenous steroids without prior gonadotrophin-releasing hormone agonist suppression yields favourable pregnancy results.Hum Reprod 12, no. 6 (June 1997): 1176–80. https://doi.org/10.1093/humrep/12.6.1176.
Journal cover image

Published In

Hum Reprod

DOI

ISSN

0268-1161

Publication Date

June 1997

Volume

12

Issue

6

Start / End Page

1176 / 1180

Location

England

Related Subject Headings

  • Time Factors
  • Retrospective Studies
  • Progesterone
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Menstrual Cycle
  • Infertility
  • Humans
  • Gonadotropin-Releasing Hormone
  • Female