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The intestinal glucose-apelin cycle controls carbohydrate absorption in mice.

Publication ,  Journal Article
Dray, C; Sakar, Y; Vinel, C; Daviaud, D; Masri, B; Garrigues, L; Wanecq, E; Galvani, S; Negre-Salvayre, A; Barak, LS; Monsarrat, B; Valet, P ...
Published in: Gastroenterology
April 2013

BACKGROUND & AIMS: Glucose is absorbed into intestine cells via the sodium glucose transporter 1 (SGLT-1) and glucose transporter 2 (GLUT2); various peptides and hormones control this process. Apelin is a peptide that regulates glucose homeostasis and is produced by proximal digestive cells; we studied whether glucose modulates apelin secretion by enterocytes and the effects of apelin on intestinal glucose absorption. METHODS: We characterized glucose-related luminal apelin secretion in vivo and ex vivo by mass spectroscopy and immunologic techniques. The effects of apelin on (14)C-labeled glucose transport were determined in jejunal loops and in mice following apelin gavage. We determined levels of GLUT2 and SGLT-1 proteins and phosphorylation of AMPKα2 by immunoblotting. The net effect of apelin on intestinal glucose transepithelial transport was determined in mice. RESULTS: Glucose stimulated luminal secretion of the pyroglutaminated apelin-13 isoform ([Pyr-1]-apelin-13) in the small intestine of mice. Apelin increased specific glucose flux through the gastric epithelial barrier in jejunal loops and in vivo following oral glucose administration. Conversely, pharmacologic apelin blockade in the intestine reduced the increased glycemia that occurs following oral glucose administration. Apelin activity was associated with phosphorylation of AMPKα2 and a rapid increase of the GLUT2/SGLT-1 protein ratio in the brush border membrane. CONCLUSIONS: Glucose amplifies its own transport from the intestinal lumen to the bloodstream by increasing luminal apelin secretion. In the lumen, active apelin regulates carbohydrate flux through enterocytes by promoting AMPKα2 phosphorylation and modifying the ratio of SGLT-1:GLUT2. The glucose-apelin cycle might be pharmacologically handled to regulate glucose absorption and assess better control of glucose homeostasis.

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Published In

Gastroenterology

DOI

EISSN

1528-0012

Publication Date

April 2013

Volume

144

Issue

4

Start / End Page

771 / 780

Location

United States

Related Subject Headings

  • Sodium-Glucose Transporter 1
  • Reference Values
  • Random Allocation
  • Microscopy, Confocal
  • Mice, Inbred C57BL
  • Mice
  • Mass Spectrometry
  • Male
  • Intestinal Absorption
  • Intercellular Signaling Peptides and Proteins
 

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Dray, C., Sakar, Y., Vinel, C., Daviaud, D., Masri, B., Garrigues, L., … Ducroc, R. (2013). The intestinal glucose-apelin cycle controls carbohydrate absorption in mice. Gastroenterology, 144(4), 771–780. https://doi.org/10.1053/j.gastro.2013.01.004
Dray, Cédric, Yassine Sakar, Claire Vinel, Daniele Daviaud, Bernard Masri, Luc Garrigues, Estelle Wanecq, et al. “The intestinal glucose-apelin cycle controls carbohydrate absorption in mice.Gastroenterology 144, no. 4 (April 2013): 771–80. https://doi.org/10.1053/j.gastro.2013.01.004.
Dray C, Sakar Y, Vinel C, Daviaud D, Masri B, Garrigues L, et al. The intestinal glucose-apelin cycle controls carbohydrate absorption in mice. Gastroenterology. 2013 Apr;144(4):771–80.
Dray, Cédric, et al. “The intestinal glucose-apelin cycle controls carbohydrate absorption in mice.Gastroenterology, vol. 144, no. 4, Apr. 2013, pp. 771–80. Pubmed, doi:10.1053/j.gastro.2013.01.004.
Dray C, Sakar Y, Vinel C, Daviaud D, Masri B, Garrigues L, Wanecq E, Galvani S, Negre-Salvayre A, Barak LS, Monsarrat B, Burlet-Schiltz O, Valet P, Castan-Laurell I, Ducroc R. The intestinal glucose-apelin cycle controls carbohydrate absorption in mice. Gastroenterology. 2013 Apr;144(4):771–780.
Journal cover image

Published In

Gastroenterology

DOI

EISSN

1528-0012

Publication Date

April 2013

Volume

144

Issue

4

Start / End Page

771 / 780

Location

United States

Related Subject Headings

  • Sodium-Glucose Transporter 1
  • Reference Values
  • Random Allocation
  • Microscopy, Confocal
  • Mice, Inbred C57BL
  • Mice
  • Mass Spectrometry
  • Male
  • Intestinal Absorption
  • Intercellular Signaling Peptides and Proteins