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Attenuation of store-operated Ca2+ current impairs salivary gland fluid secretion in TRPC1(-/-) mice.

Publication ,  Journal Article
Liu, X; Cheng, KT; Bandyopadhyay, BC; Pani, B; Dietrich, A; Paria, BC; Swaim, WD; Beech, D; Yildrim, E; Singh, BB; Birnbaumer, L; Ambudkar, IS
Published in: Proc Natl Acad Sci U S A
October 30, 2007

Agonist-induced Ca(2+) entry via store-operated Ca(2+) (SOC) channels is suggested to regulate a wide variety of cellular functions, including salivary gland fluid secretion. However, the molecular components of these channels and their physiological function(s) are largely unknown. Here we report that attenuation of SOC current underlies salivary gland dysfunction in mice lacking transient receptor potential 1 (TRPC1). Neurotransmitter-regulated salivary gland fluid secretion in TRPC1-deficient TRPC1(-/-) mice was severely decreased (by 70%). Further, agonist- and thapsigargin-stimulated SOC channel activity was significantly reduced in salivary gland acinar cells isolated from TRPC1(-/-) mice. Deletion of TRPC1 also eliminated sustained Ca(2+)-dependent potassium channel activity, which depends on Ca(2+) entry and is required for fluid secretion. Expression of key proteins involved in fluid secretion and Ca(2+) signaling, including STIM1 and other TRPC channels, was not altered. Together, these data demonstrate that reduced SOC entry accounts for the severe loss of salivary gland fluid secretion in TRPC1(-/-) mice. Thus, TRPC1 is a critical component of the SOC channel in salivary gland acinar cells and is essential for neurotransmitter-regulation of fluid secretion.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 30, 2007

Volume

104

Issue

44

Start / End Page

17542 / 17547

Location

United States

Related Subject Headings

  • TRPC Cation Channels
  • Salivary Glands
  • Saliva
  • Potassium Channels
  • Mice, Knockout
  • Mice
  • Gene Expression Regulation
  • Calcium
  • Animals
 

Citation

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Liu, X., Cheng, K. T., Bandyopadhyay, B. C., Pani, B., Dietrich, A., Paria, B. C., … Ambudkar, I. S. (2007). Attenuation of store-operated Ca2+ current impairs salivary gland fluid secretion in TRPC1(-/-) mice. Proc Natl Acad Sci U S A, 104(44), 17542–17547. https://doi.org/10.1073/pnas.0701254104
Liu, Xibao, Kwong Tai Cheng, Bidhan C. Bandyopadhyay, Biswaranjan Pani, Alexander Dietrich, Biman C. Paria, William D. Swaim, et al. “Attenuation of store-operated Ca2+ current impairs salivary gland fluid secretion in TRPC1(-/-) mice.Proc Natl Acad Sci U S A 104, no. 44 (October 30, 2007): 17542–47. https://doi.org/10.1073/pnas.0701254104.
Liu X, Cheng KT, Bandyopadhyay BC, Pani B, Dietrich A, Paria BC, et al. Attenuation of store-operated Ca2+ current impairs salivary gland fluid secretion in TRPC1(-/-) mice. Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17542–7.
Liu, Xibao, et al. “Attenuation of store-operated Ca2+ current impairs salivary gland fluid secretion in TRPC1(-/-) mice.Proc Natl Acad Sci U S A, vol. 104, no. 44, Oct. 2007, pp. 17542–47. Pubmed, doi:10.1073/pnas.0701254104.
Liu X, Cheng KT, Bandyopadhyay BC, Pani B, Dietrich A, Paria BC, Swaim WD, Beech D, Yildrim E, Singh BB, Birnbaumer L, Ambudkar IS. Attenuation of store-operated Ca2+ current impairs salivary gland fluid secretion in TRPC1(-/-) mice. Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17542–17547.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 30, 2007

Volume

104

Issue

44

Start / End Page

17542 / 17547

Location

United States

Related Subject Headings

  • TRPC Cation Channels
  • Salivary Glands
  • Saliva
  • Potassium Channels
  • Mice, Knockout
  • Mice
  • Gene Expression Regulation
  • Calcium
  • Animals