A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.
Publication
, Journal Article
Eroglu, C; Yildiz, E; Ozturk, M; Pinarbasi, E
Published in: Acta Virol
February 2000
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3rd generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.
Duke Scholars
Published In
Acta Virol
ISSN
0001-723X
Publication Date
February 2000
Volume
44
Issue
1
Start / End Page
29 / 33
Location
Slovakia
Related Subject Headings
- Virology
- Viral Core Proteins
- Serologic Tests
- Sensitivity and Specificity
- Recombinant Proteins
- Humans
- Hepatitis C Antigens
- Hepatitis C Antibodies
- Hepatitis C
- Hepacivirus
Citation
APA
Chicago
ICMJE
MLA
NLM
Eroglu, C., Yildiz, E., Ozturk, M., & Pinarbasi, E. (2000). A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus. Acta Virol, 44(1), 29–33.
Eroglu, C., E. Yildiz, M. Ozturk, and E. Pinarbasi. “A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.” Acta Virol 44, no. 1 (February 2000): 29–33.
Eroglu C, Yildiz E, Ozturk M, Pinarbasi E. A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus. Acta Virol. 2000 Feb;44(1):29–33.
Eroglu, C., et al. “A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.” Acta Virol, vol. 44, no. 1, Feb. 2000, pp. 29–33.
Eroglu C, Yildiz E, Ozturk M, Pinarbasi E. A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus. Acta Virol. 2000 Feb;44(1):29–33.
Published In
Acta Virol
ISSN
0001-723X
Publication Date
February 2000
Volume
44
Issue
1
Start / End Page
29 / 33
Location
Slovakia
Related Subject Headings
- Virology
- Viral Core Proteins
- Serologic Tests
- Sensitivity and Specificity
- Recombinant Proteins
- Humans
- Hepatitis C Antigens
- Hepatitis C Antibodies
- Hepatitis C
- Hepacivirus