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Prenatal hypoxia decreases lung extracellular superoxide dismutase expression and activity.

Publication ,  Journal Article
Giles, B-L; Suliman, H; Mamo, LB; Piantadosi, CA; Oury, TD; Nozik-Grayck, E
Published in: Am J Physiol Lung Cell Mol Physiol
September 2002

Extracellular superoxide dismutase (EC-SOD), which scavenges extracellular superoxide (O.), is highly regulated in the developing lung. In the prenatal rabbit, EC-SOD is predominantly intracellular and inactive, and postnatally, active EC-SOD is secreted. We hypothesized that prenatal hypoxia would delay the normal postnatal secretion of active EC-SOD in the lung. Pregnant New Zealand White rabbits were exposed to hypobaric hypoxia (15,000 ft x 36 h) to alter fetal O(2) tension or were maintained in room air. Lungs were harvested from preterm (28 days), term (30 +/- 1 day), and 1-wk-old kits. After prenatal hypobaric hypoxia, EC-SOD mRNA expression was significantly decreased in lungs of full-term kits, whereas EC-SOD protein decreased at all ages. Immunohistochemical staining for EC-SOD showed that hypoxia delayed secretion of the isoenzyme in the airways and pulmonary vasculature. Furthermore, pulmonary EC-SOD enzyme activity was significantly decreased in the 1-wk-old kits exposed to prenatal hypoxia. We conclude that prenatal hypoxia downregulates EC-SOD expression at both the transcriptional and posttranslational levels. Furthermore, prenatal hypoxia delays secretion of active EC-SOD enzyme. These findings have important implications for the effects of prenatal asphyxia on postnatal response to oxidant stress.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

September 2002

Volume

283

Issue

3

Start / End Page

L549 / L554

Location

United States

Related Subject Headings

  • Superoxide Dismutase
  • Respiratory System
  • Rabbits
  • RNA, Messenger
  • Pregnancy
  • Lung
  • Hypoxia
  • Gestational Age
  • Female
  • Extracellular Space
 

Citation

APA
Chicago
ICMJE
MLA
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Giles, B.-L., Suliman, H., Mamo, L. B., Piantadosi, C. A., Oury, T. D., & Nozik-Grayck, E. (2002). Prenatal hypoxia decreases lung extracellular superoxide dismutase expression and activity. Am J Physiol Lung Cell Mol Physiol, 283(3), L549–L554. https://doi.org/10.1152/ajplung.00018.2002
Giles, Brenda-Louise, Hagir Suliman, Lisa B. Mamo, Claude A. Piantadosi, Tim D. Oury, and Eva Nozik-Grayck. “Prenatal hypoxia decreases lung extracellular superoxide dismutase expression and activity.Am J Physiol Lung Cell Mol Physiol 283, no. 3 (September 2002): L549–54. https://doi.org/10.1152/ajplung.00018.2002.
Giles B-L, Suliman H, Mamo LB, Piantadosi CA, Oury TD, Nozik-Grayck E. Prenatal hypoxia decreases lung extracellular superoxide dismutase expression and activity. Am J Physiol Lung Cell Mol Physiol. 2002 Sep;283(3):L549–54.
Giles, Brenda-Louise, et al. “Prenatal hypoxia decreases lung extracellular superoxide dismutase expression and activity.Am J Physiol Lung Cell Mol Physiol, vol. 283, no. 3, Sept. 2002, pp. L549–54. Pubmed, doi:10.1152/ajplung.00018.2002.
Giles B-L, Suliman H, Mamo LB, Piantadosi CA, Oury TD, Nozik-Grayck E. Prenatal hypoxia decreases lung extracellular superoxide dismutase expression and activity. Am J Physiol Lung Cell Mol Physiol. 2002 Sep;283(3):L549–L554.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

September 2002

Volume

283

Issue

3

Start / End Page

L549 / L554

Location

United States

Related Subject Headings

  • Superoxide Dismutase
  • Respiratory System
  • Rabbits
  • RNA, Messenger
  • Pregnancy
  • Lung
  • Hypoxia
  • Gestational Age
  • Female
  • Extracellular Space