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Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation.

Publication ,  Journal Article
Baka, SG; Toth, TL; Veeck, LL; Jones, HW; Muasher, SJ; Lanzendorf, SE
Published in: Hum Reprod
July 1995

The present study was conducted to determine if the cryopreservation of immature human oocytes has a deleterious effect on the meiotic spindle following maturation in vitro. Oocytes were obtained in excess from in-vitro fertilization patients and divided into four groups. Groups 1 (n = 98) and 2 (n = 80) consisted of immature oocytes cryopreserved before or after maturation in vitro respectively. Groups 3 (n = 37) and 4 (n = 9) served as non-frozen controls and included oocytes matured in vitro and in vivo respectively. The meiotic spindle was identified after incubation in anti-tubulin monoclonal antibody (1 h, 37 degrees C) and fluorescein-conjugated goat anti-mouse immunoglobulin G (IgG) (1 h, RT). Chromosomes were counterstained with 4',6'-diamidino-2-phenylindole. Following cryopreservation, group 1 oocytes demonstrated a 63% survival rate and 68% maturation rate in vitro. In all, 58% of the oocytes in group 2 survived the thaw. The number of oocytes with normal spindles in group 1 (81.0%) was not significantly different from control groups 3 (83.8%) and 4 (88.9%), while the number of group 2 oocytes with normal structures (43.5%) was significantly lower than groups 1 (P = 0.0004), 3 (P = 0.0002), and 4 (P = 0.025). These results suggest that cryopreservation of the prophase I human oocyte does not significantly increase abnormalities in the resulting meiotic spindle.

Duke Scholars

Published In

Hum Reprod

DOI

ISSN

0268-1161

Publication Date

July 1995

Volume

10

Issue

7

Start / End Page

1816 / 1820

Location

England

Related Subject Headings

  • Spindle Apparatus
  • Oocytes
  • Obstetrics & Reproductive Medicine
  • Humans
  • Fluorescent Antibody Technique
  • Cryopreservation
  • Chromosomes
  • Cellular Senescence
  • Cells, Cultured
  • Cell Survival
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Baka, S. G., Toth, T. L., Veeck, L. L., Jones, H. W., Muasher, S. J., & Lanzendorf, S. E. (1995). Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation. Hum Reprod, 10(7), 1816–1820. https://doi.org/10.1093/oxfordjournals.humrep.a136182
Baka, S. G., T. L. Toth, L. L. Veeck, H. W. Jones, S. J. Muasher, and S. E. Lanzendorf. “Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation.Hum Reprod 10, no. 7 (July 1995): 1816–20. https://doi.org/10.1093/oxfordjournals.humrep.a136182.
Baka SG, Toth TL, Veeck LL, Jones HW, Muasher SJ, Lanzendorf SE. Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation. Hum Reprod. 1995 Jul;10(7):1816–20.
Baka, S. G., et al. “Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation.Hum Reprod, vol. 10, no. 7, July 1995, pp. 1816–20. Pubmed, doi:10.1093/oxfordjournals.humrep.a136182.
Baka SG, Toth TL, Veeck LL, Jones HW, Muasher SJ, Lanzendorf SE. Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation. Hum Reprod. 1995 Jul;10(7):1816–1820.
Journal cover image

Published In

Hum Reprod

DOI

ISSN

0268-1161

Publication Date

July 1995

Volume

10

Issue

7

Start / End Page

1816 / 1820

Location

England

Related Subject Headings

  • Spindle Apparatus
  • Oocytes
  • Obstetrics & Reproductive Medicine
  • Humans
  • Fluorescent Antibody Technique
  • Cryopreservation
  • Chromosomes
  • Cellular Senescence
  • Cells, Cultured
  • Cell Survival