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Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum.

Publication ,  Journal Article
Lai, FA; Anderson, K; Rousseau, E; Liu, QY; Meissner, G
Published in: Biochem Biophys Res Commun
February 29, 1988

The solubilized [3H]ryanodine receptor from cardiac sarcoplasmic reticulum was centrifuged through linear sucrose gradients. A single peak of radioactivity with apparent sedimentation coefficient of approximately 30S specifically comigrated with a high molecular weight protein of apparent relative molecular mass approximately 400,000. Incorporation of the ryanodine receptor into lipid bilayers induced single Ca2+ channel currents with conductance and kinetic behavior almost identical to that of native cardiac Ca2+ release channels. These results suggest that the cardiac ryanodine receptor comprises the Ca2+ release channel involved in excitation-contraction coupling in cardiac muscle.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

February 29, 1988

Volume

151

Issue

1

Start / End Page

441 / 449

Location

United States

Related Subject Headings

  • Sarcoplasmic Reticulum
  • Ryanodine Receptor Calcium Release Channel
  • Receptors, Cholinergic
  • Myocardium
  • Molecular Weight
  • Ion Channels
  • Electrophoresis, Polyacrylamide Gel
  • Dogs
  • Centrifugation, Density Gradient
  • Calcium
 

Citation

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MLA
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Lai, F. A., Anderson, K., Rousseau, E., Liu, Q. Y., & Meissner, G. (1988). Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum. Biochem Biophys Res Commun, 151(1), 441–449. https://doi.org/10.1016/0006-291x(88)90613-4
Lai, F. A., K. Anderson, E. Rousseau, Q. Y. Liu, and G. Meissner. “Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum.Biochem Biophys Res Commun 151, no. 1 (February 29, 1988): 441–49. https://doi.org/10.1016/0006-291x(88)90613-4.
Lai FA, Anderson K, Rousseau E, Liu QY, Meissner G. Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum. Biochem Biophys Res Commun. 1988 Feb 29;151(1):441–9.
Lai, F. A., et al. “Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum.Biochem Biophys Res Commun, vol. 151, no. 1, Feb. 1988, pp. 441–49. Pubmed, doi:10.1016/0006-291x(88)90613-4.
Lai FA, Anderson K, Rousseau E, Liu QY, Meissner G. Evidence for a Ca2+ channel within the ryanodine receptor complex from cardiac sarcoplasmic reticulum. Biochem Biophys Res Commun. 1988 Feb 29;151(1):441–449.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

February 29, 1988

Volume

151

Issue

1

Start / End Page

441 / 449

Location

United States

Related Subject Headings

  • Sarcoplasmic Reticulum
  • Ryanodine Receptor Calcium Release Channel
  • Receptors, Cholinergic
  • Myocardium
  • Molecular Weight
  • Ion Channels
  • Electrophoresis, Polyacrylamide Gel
  • Dogs
  • Centrifugation, Density Gradient
  • Calcium