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LexA protein of Escherichia coli represses expression of the Tn5 transposase gene.

Publication ,  Journal Article
Kuan, CT; Tessman, I
Published in: J Bacteriol
October 1991

The LexA protein of Escherichia coli represses expression of a variety of genes that, by definition, constitute the SOS regulon. Genetic evidence suggests that Tn5 transposition is also regulated by the product of the lexA gene (C.-T. Kuan, S.-K. Liu, and I. Tessman, Genetics 128:45-57, 1991). We now show that the LexA protein represses expression of the tnp gene, located in the IS50R component of Tn5, which encodes a transposase, and that LexA does not repress expression of the IS50R inh gene, which encodes an inhibitor of transposition. Elimination of LexA resulted in increased expression of the tnp gene by a factor of 2.7 +/- 0.4, as indicated by the activity of a lacZ gene fused to the tnp gene. LexA protein retarded the electrophoretic movement of a 101-bp segment of IS50R DNA that contained a putative LexA protein-binding site in the tnp promoter; the interaction between the LexA repressor and the promoter region of the tnp gene appears to be relatively weak. These features show that the IS50R tnp gene is a member of the SOS regulon.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

October 1991

Volume

173

Issue

20

Start / End Page

6406 / 6410

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Transposases
  • Serine Endopeptidases
  • SOS Response, Genetics
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Nucleotidyltransferases
  • Molecular Sequence Data
  • Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
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Kuan, C. T., & Tessman, I. (1991). LexA protein of Escherichia coli represses expression of the Tn5 transposase gene. J Bacteriol, 173(20), 6406–6410. https://doi.org/10.1128/jb.173.20.6406-6410.1991
Kuan, C. T., and I. Tessman. “LexA protein of Escherichia coli represses expression of the Tn5 transposase gene.J Bacteriol 173, no. 20 (October 1991): 6406–10. https://doi.org/10.1128/jb.173.20.6406-6410.1991.
Kuan CT, Tessman I. LexA protein of Escherichia coli represses expression of the Tn5 transposase gene. J Bacteriol. 1991 Oct;173(20):6406–10.
Kuan, C. T., and I. Tessman. “LexA protein of Escherichia coli represses expression of the Tn5 transposase gene.J Bacteriol, vol. 173, no. 20, Oct. 1991, pp. 6406–10. Pubmed, doi:10.1128/jb.173.20.6406-6410.1991.
Kuan CT, Tessman I. LexA protein of Escherichia coli represses expression of the Tn5 transposase gene. J Bacteriol. 1991 Oct;173(20):6406–6410.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

October 1991

Volume

173

Issue

20

Start / End Page

6406 / 6410

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Transposases
  • Serine Endopeptidases
  • SOS Response, Genetics
  • Recombinant Fusion Proteins
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • Nucleotidyltransferases
  • Molecular Sequence Data
  • Microbiology