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Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics.

Publication ,  Journal Article
Rancz, EA; Franks, KM; Schwarz, MK; Pichler, B; Schaefer, AT; Margrie, TW
Published in: Nat Neurosci
April 2011

Single-cell genetic manipulation is expected to substantially advance the field of systems neuroscience. However, existing gene delivery techniques do not allow researchers to electrophysiologically characterize cells and to thereby establish an experimental link between physiology and genetics for understanding neuronal function. In the mouse brain in vivo, we found that neurons remained intact after 'blind' whole-cell recording, that DNA vectors could be delivered through the patch-pipette during such recordings and that these vectors drove protein expression in recorded cells for at least 7 d. To illustrate the utility of this approach, we recorded visually evoked synaptic responses in primary visual cortical cells while delivering DNA plasmids that allowed retrograde, monosynaptic tracing of each neuron's presynaptic inputs. By providing a biophysical profile of a cell before its specific genetic perturbation, this combinatorial method captures the synaptic and anatomical receptive field of a neuron.

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Published In

Nat Neurosci

DOI

EISSN

1546-1726

Publication Date

April 2011

Volume

14

Issue

4

Start / End Page

527 / 532

Location

United States

Related Subject Headings

  • Transfection
  • Patch-Clamp Techniques
  • Organ Culture Techniques
  • Neurons
  • Neurology & Neurosurgery
  • Neuroanatomical Tract-Tracing Techniques
  • Mice, Inbred C57BL
  • Mice
  • Genetic Vectors
  • Brain
 

Citation

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Rancz, E. A., Franks, K. M., Schwarz, M. K., Pichler, B., Schaefer, A. T., & Margrie, T. W. (2011). Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics. Nat Neurosci, 14(4), 527–532. https://doi.org/10.1038/nn.2765
Rancz, Ede A., Kevin M. Franks, Martin K. Schwarz, Bruno Pichler, Andreas T. Schaefer, and Troy W. Margrie. “Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics.Nat Neurosci 14, no. 4 (April 2011): 527–32. https://doi.org/10.1038/nn.2765.
Rancz EA, Franks KM, Schwarz MK, Pichler B, Schaefer AT, Margrie TW. Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics. Nat Neurosci. 2011 Apr;14(4):527–32.
Rancz, Ede A., et al. “Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics.Nat Neurosci, vol. 14, no. 4, Apr. 2011, pp. 527–32. Pubmed, doi:10.1038/nn.2765.
Rancz EA, Franks KM, Schwarz MK, Pichler B, Schaefer AT, Margrie TW. Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics. Nat Neurosci. 2011 Apr;14(4):527–532.

Published In

Nat Neurosci

DOI

EISSN

1546-1726

Publication Date

April 2011

Volume

14

Issue

4

Start / End Page

527 / 532

Location

United States

Related Subject Headings

  • Transfection
  • Patch-Clamp Techniques
  • Organ Culture Techniques
  • Neurons
  • Neurology & Neurosurgery
  • Neuroanatomical Tract-Tracing Techniques
  • Mice, Inbred C57BL
  • Mice
  • Genetic Vectors
  • Brain