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Novel function of clathrin light chain in promoting endocytic vesicle formation.

Publication ,  Journal Article
Newpher, TM; Idrissi, F-Z; Geli, MI; Lemmon, SK
Published in: Molecular biology of the cell
October 2006

Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts with members of the Hip1/R family of endocytic proteins, including the budding yeast homologue, Sla2p. Here, using in vivo analysis in yeast, we provide novel insight into the role of this interaction. We find that overexpression of LC partially restores endocytosis to cells lacking clathrin HC. This suppression is dependent on the Sla2p binding region of LC. Using live cell imaging techniques to visualize endocytic vesicle formation, we find that the N-terminal Sla2p binding region of LC promotes the progression of arrested Sla2p patches that form in the absence of HC. We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation.

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Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

October 2006

Volume

17

Issue

10

Start / End Page

4343 / 4352

Related Subject Headings

  • Transport Vesicles
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Models, Biological
  • Developmental Biology
  • Cytoskeletal Proteins
  • Clathrin-Coated Vesicles
  • Clathrin Light Chains
  • Clathrin Heavy Chains
  • Carrier Proteins
 

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Newpher, T. M., Idrissi, F.-Z., Geli, M. I., & Lemmon, S. K. (2006). Novel function of clathrin light chain in promoting endocytic vesicle formation. Molecular Biology of the Cell, 17(10), 4343–4352. https://doi.org/10.1091/mbc.e06-07-0606
Newpher, Thomas M., Fatima-Zahra Idrissi, Maria Isabel Geli, and Sandra K. Lemmon. “Novel function of clathrin light chain in promoting endocytic vesicle formation.Molecular Biology of the Cell 17, no. 10 (October 2006): 4343–52. https://doi.org/10.1091/mbc.e06-07-0606.
Newpher TM, Idrissi F-Z, Geli MI, Lemmon SK. Novel function of clathrin light chain in promoting endocytic vesicle formation. Molecular biology of the cell. 2006 Oct;17(10):4343–52.
Newpher, Thomas M., et al. “Novel function of clathrin light chain in promoting endocytic vesicle formation.Molecular Biology of the Cell, vol. 17, no. 10, Oct. 2006, pp. 4343–52. Epmc, doi:10.1091/mbc.e06-07-0606.
Newpher TM, Idrissi F-Z, Geli MI, Lemmon SK. Novel function of clathrin light chain in promoting endocytic vesicle formation. Molecular biology of the cell. 2006 Oct;17(10):4343–4352.

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

October 2006

Volume

17

Issue

10

Start / End Page

4343 / 4352

Related Subject Headings

  • Transport Vesicles
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Models, Biological
  • Developmental Biology
  • Cytoskeletal Proteins
  • Clathrin-Coated Vesicles
  • Clathrin Light Chains
  • Clathrin Heavy Chains
  • Carrier Proteins