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Glutamine metabolism during aerial mycelium growth of Neurospora crassa.

Publication ,  Journal Article
Cárdenas, ME; Hansberg, W
Published in: J Gen Microbiol
July 1984

During vegetative growth, glutamine is accumulated in the mycelium of Neurospora crassa. This high pool of glutamine seems to be required for aerial mycelium growth. Enzymes responsible for the synthesis and catabolism of glutamine were measured before and during the partial transformation of a mycelial mat into aerial mycelium. In the transforming mycelial mat,considerable activities of the biosynthetic NADP-glutamate dehydrogenase and glutamine synthetase (predominantly β polypeptide) and also some activity of glutamate synthase were observed. In the aerial mycelium, glutamine synthetase (predominantly β polypeptide) was detected, but very low activities of NADP-glutamate dehydrogenase and glutamate mycelium could derive from glutamine. No glutaminase activity could be detected. It is suggested that glutamate is formed through the activities of the glutamine transaminase-ω -amidase pathway and another transaminase. High activities of glutamine and alanine transaminases were observed in the aerial mycelium. These results are discussed in terms of the possible role of glutamine as a nitrogen carrier from the mycelium to the growing aerial hyphae.

Duke Scholars

Published In

J Gen Microbiol

DOI

ISSN

0022-1287

Publication Date

July 1984

Volume

130

Issue

7

Start / End Page

1733 / 1741

Location

England

Related Subject Headings

  • Transaminases
  • Neurospora crassa
  • Mycelium
  • Metabolic Networks and Pathways
  • Glutamine
  • Glutaminase
  • Glutamic Acid
  • Glutamate-Ammonia Ligase
  • Glutamate Synthase
  • Glutamate Dehydrogenase (NADP+)
 

Citation

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Cárdenas, M. E., & Hansberg, W. (1984). Glutamine metabolism during aerial mycelium growth of Neurospora crassa. J Gen Microbiol, 130(7), 1733–1741. https://doi.org/10.1099/00221287-130-7-1733
Cárdenas, M. E., and W. Hansberg. “Glutamine metabolism during aerial mycelium growth of Neurospora crassa.J Gen Microbiol 130, no. 7 (July 1984): 1733–41. https://doi.org/10.1099/00221287-130-7-1733.
Cárdenas ME, Hansberg W. Glutamine metabolism during aerial mycelium growth of Neurospora crassa. J Gen Microbiol. 1984 Jul;130(7):1733–41.
Cárdenas, M. E., and W. Hansberg. “Glutamine metabolism during aerial mycelium growth of Neurospora crassa.J Gen Microbiol, vol. 130, no. 7, July 1984, pp. 1733–41. Pubmed, doi:10.1099/00221287-130-7-1733.
Cárdenas ME, Hansberg W. Glutamine metabolism during aerial mycelium growth of Neurospora crassa. J Gen Microbiol. 1984 Jul;130(7):1733–1741.

Published In

J Gen Microbiol

DOI

ISSN

0022-1287

Publication Date

July 1984

Volume

130

Issue

7

Start / End Page

1733 / 1741

Location

England

Related Subject Headings

  • Transaminases
  • Neurospora crassa
  • Mycelium
  • Metabolic Networks and Pathways
  • Glutamine
  • Glutaminase
  • Glutamic Acid
  • Glutamate-Ammonia Ligase
  • Glutamate Synthase
  • Glutamate Dehydrogenase (NADP+)