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Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip.

Publication ,  Journal Article
Ngo, HT; Wang, H-N; Fales, AM; Vo-Dinh, T
Published in: Analytical chemistry
July 2013

Development of a rapid, cost-effective, label-free biosensor for DNA detection is important for many applications in clinical diagnosis, homeland defense, and environment monitoring. A unique label-free DNA biosensor based on Molecular Sentinel (MS) immobilized on a plasmonic 'Nanowave' chip, which is also referred to as a metal film over nanosphere (MFON), is presented. Its sensing mechanism is based upon the decrease of the surface-enhanced Raman scattering (SERS) intensity when Raman label tagged at one end of MS is physically separated from the MFON's surface upon DNA hybridization. This method is label-free as the target does not have to be labeled. The MFON fabrication is relatively simple and low-cost with high reproducibility based on depositing a thin shell of gold over close-packed arrays of nanospheres. The sensing process involves a single hybridization step between the DNA target sequences and the complementary MS probes on the Nanowave chip without requiring secondary hybridization or posthybridization washing, thus resulting in rapid assay time and low reagent usage. The usefulness and potential application of the biosensor for medical diagnostics is demonstrated by detecting the human radical S-adenosyl methionine domain containing 2 (RSAD2) gene, a common inflammation biomarker.

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Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

July 2013

Volume

85

Issue

13

Start / End Page

6378 / 6383

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Proteins
  • Oxidoreductases Acting on CH-CH Group Donors
  • Nanotechnology
  • Humans
  • DNA
  • Biosensing Techniques
  • Analytical Chemistry
  • 4004 Chemical engineering
 

Citation

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Ngo, H. T., Wang, H.-N., Fales, A. M., & Vo-Dinh, T. (2013). Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip. Analytical Chemistry, 85(13), 6378–6383. https://doi.org/10.1021/ac400763c
Ngo, Hoan Thanh, Hsin-Neng Wang, Andrew M. Fales, and Tuan Vo-Dinh. “Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip.Analytical Chemistry 85, no. 13 (July 2013): 6378–83. https://doi.org/10.1021/ac400763c.
Ngo HT, Wang H-N, Fales AM, Vo-Dinh T. Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip. Analytical chemistry. 2013 Jul;85(13):6378–83.
Ngo, Hoan Thanh, et al. “Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip.Analytical Chemistry, vol. 85, no. 13, July 2013, pp. 6378–83. Epmc, doi:10.1021/ac400763c.
Ngo HT, Wang H-N, Fales AM, Vo-Dinh T. Label-free DNA biosensor based on SERS Molecular Sentinel on Nanowave chip. Analytical chemistry. 2013 Jul;85(13):6378–6383.
Journal cover image

Published In

Analytical chemistry

DOI

EISSN

1520-6882

ISSN

0003-2700

Publication Date

July 2013

Volume

85

Issue

13

Start / End Page

6378 / 6383

Related Subject Headings

  • Surface Properties
  • Spectrum Analysis, Raman
  • Proteins
  • Oxidoreductases Acting on CH-CH Group Donors
  • Nanotechnology
  • Humans
  • DNA
  • Biosensing Techniques
  • Analytical Chemistry
  • 4004 Chemical engineering