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Development of antibody-based fiber-optic sensors

Publication ,  Journal Article
Tromberg, BJ; Sepaniak, MJ; Vo-Dinh, T
Published in: Proceedings of SPIE - The International Society for Optical Engineering
June 21, 1988

The speed and specificity characteristic of immunochemical complex formation has encouraged the development of numerous antibody-based analytical techniques. The scope and versatility of these established methods can be enhanced by combining the principles of conventional immunoassay with laser-based fiber-optic fluorimetry. This merger of spectroscopy and immunochemistry provides the framework for the construction of highly sensitive and selective fiber-optic devices (fluoroimmuno-sensors) capable of in-situ detection of drugs, toxins, and naturally occurring biochemicals. Fluoroimmuno-sensors (FIS) employ an immobilized reagent phase at the sampling terminus of a single quartz optical fiber. Laser excitation of antibody-bound analyte produces a fluorescence signal which is either directly proportional (as in the case of natural fluorophor and ‘antibody sandwich’ assays) or inversely proportional (as in the case of competitive-binding assays) to analyte concentration. Factors which influence analysis time, precision, linearity, and detection limits include the nature (solid or liquid) and amount of the reagent phase, the method of analyte delivery (passive diffusion, convection, etc.), and whether equilibrium or non-equilibrium assays are performed. Data will be presented for optical fibers whose sensing termini utilize: (1) covalently-bound solid antibody reagent phases, and (2) membrane-entrapped liquid antibody reagents. Assays for large-molecular weight proteins (antigens) and small-molecular weight, carcinogenic, polynuclear aromatics (haptens) will be considered. In this manner, the influence of a system's chemical characteristics and measurement requirements on sensor design, and the consequence of various sensor designs on analytical performance will be illustrated. © 1988 SPIE.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

June 21, 1988

Volume

906

Start / End Page

30 / 38

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

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Tromberg, B. J., Sepaniak, M. J., & Vo-Dinh, T. (1988). Development of antibody-based fiber-optic sensors. Proceedings of SPIE - The International Society for Optical Engineering, 906, 30–38. https://doi.org/10.1117/12.945251
Tromberg, B. J., M. J. Sepaniak, and T. Vo-Dinh. “Development of antibody-based fiber-optic sensors.” Proceedings of SPIE - The International Society for Optical Engineering 906 (June 21, 1988): 30–38. https://doi.org/10.1117/12.945251.
Tromberg BJ, Sepaniak MJ, Vo-Dinh T. Development of antibody-based fiber-optic sensors. Proceedings of SPIE - The International Society for Optical Engineering. 1988 Jun 21;906:30–8.
Tromberg, B. J., et al. “Development of antibody-based fiber-optic sensors.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 906, June 1988, pp. 30–38. Scopus, doi:10.1117/12.945251.
Tromberg BJ, Sepaniak MJ, Vo-Dinh T. Development of antibody-based fiber-optic sensors. Proceedings of SPIE - The International Society for Optical Engineering. 1988 Jun 21;906:30–38.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

EISSN

1996-756X

ISSN

0277-786X

Publication Date

June 21, 1988

Volume

906

Start / End Page

30 / 38

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering