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Fast endocytosis is inhibited by GABA-mediated chloride influx at a presynaptic terminal.

Publication ,  Journal Article
Hull, C; von Gersdorff, H
Published in: Neuron
October 28, 2004

Although multiple kinetic components of synaptic vesicle endocytosis have been identified, it has remained unclear whether neurons can differentially modulate these components. Using membrane capacitance measurements from isolated goldfish bipolar cell terminals, we found that the kinetics of endocytosis in retinal slices (single exponential decay; tau > 10 s) were significantly slower than those in acutely dissociated terminals (double exponential decay; tau(fast) approximately 1-2 s; tau(slow) > 10 s). Surprisingly, GABA(A) and/or GABA(C) receptor antagonists restored the fast component of endocytosis to terminals in retinal slices. Blocking GABAergic feedback from reciprocal synapses or removing external Cl(-) ions also allowed for fast endocytosis. Elevating internal Cl(-) via the patch pipette invariably slowed endocytosis, even in terminals dialyzed with increased Ca(2+) buffer. These results suggest a new role for GABA and Cl(-) ions in blocking the trigger for fast endocytosis at this ribbon-type synapse.

Duke Scholars

Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

October 28, 2004

Volume

44

Issue

3

Start / End Page

469 / 482

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Valine
  • Time Factors
  • Retina
  • Quinoxalines
  • Pyridines
  • Presynaptic Terminals
  • Picrotoxin
  • Phosphinic Acids
  • Patch-Clamp Techniques
 

Citation

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MLA
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Hull, C., & von Gersdorff, H. (2004). Fast endocytosis is inhibited by GABA-mediated chloride influx at a presynaptic terminal. Neuron, 44(3), 469–482. https://doi.org/10.1016/j.neuron.2004.10.010
Hull, Court, and Henrique von Gersdorff. “Fast endocytosis is inhibited by GABA-mediated chloride influx at a presynaptic terminal.Neuron 44, no. 3 (October 28, 2004): 469–82. https://doi.org/10.1016/j.neuron.2004.10.010.
Hull C, von Gersdorff H. Fast endocytosis is inhibited by GABA-mediated chloride influx at a presynaptic terminal. Neuron. 2004 Oct 28;44(3):469–82.
Hull, Court, and Henrique von Gersdorff. “Fast endocytosis is inhibited by GABA-mediated chloride influx at a presynaptic terminal.Neuron, vol. 44, no. 3, Oct. 2004, pp. 469–82. Pubmed, doi:10.1016/j.neuron.2004.10.010.
Hull C, von Gersdorff H. Fast endocytosis is inhibited by GABA-mediated chloride influx at a presynaptic terminal. Neuron. 2004 Oct 28;44(3):469–482.
Journal cover image

Published In

Neuron

DOI

ISSN

0896-6273

Publication Date

October 28, 2004

Volume

44

Issue

3

Start / End Page

469 / 482

Location

United States

Related Subject Headings

  • gamma-Aminobutyric Acid
  • Valine
  • Time Factors
  • Retina
  • Quinoxalines
  • Pyridines
  • Presynaptic Terminals
  • Picrotoxin
  • Phosphinic Acids
  • Patch-Clamp Techniques