Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse.

Publication ,  Journal Article
Guatimosim, C; Hull, C; Von Gersdorff, H; Prado, MAM
Published in: J Neurochem
September 2002

Protein phosphorylation plays an essential role in regulating synaptic transmission and plasticity. However, regulation of vesicle trafficking towards and away from the plasma membrane is poorly understood. Furthermore, the extent to which phosphorylation modulates ribbon-type synapses is unknown. Using the phosphatase inhibitor okadaic acid (OA), we investigated the influence of persistent phosphorylation on vesicle cycling in goldfish bipolar cells. We followed uptake of FM1-43 during vesicle recycling in control and OA-treated cells. FM1-43 fluorescence spread to the center of control synaptic terminals after depolarization elicited Ca2+ influx. However, OA (1-50 nm) impaired this spatial spread of FM1-43 in a dose-dependent manner. Capacitance measurements revealed that OA (50 nm) did not modify either the amount or kinetics of exocytosis and endocytosis evoked by depolarizing pulses. The extremely low concentrations of OA (1-5 nm) sufficient to observe the inhibition of vesicle mobility implicate phosphatase 2A (PP2A) as a major regulator of vesicle trafficking after endocytosis. These results contrast with those at the neuromuscular junction where OA enhances lateral movement of vesicles between distinct vesicle clusters. Thus, our results suggest that phosphatases regulate vesicle translocation at ribbon synapses in a different manner than conventional active zones.

Duke Scholars

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

September 2002

Volume

82

Issue

5

Start / End Page

1047 / 1057

Location

England

Related Subject Headings

  • Synaptic Vesicles
  • Synaptic Transmission
  • Synapses
  • Retina
  • Quaternary Ammonium Compounds
  • Pyridinium Compounds
  • Patch-Clamp Techniques
  • Okadaic Acid
  • Neurology & Neurosurgery
  • In Vitro Techniques
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Guatimosim, C., Hull, C., Von Gersdorff, H., & Prado, M. A. M. (2002). Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse. J Neurochem, 82(5), 1047–1057. https://doi.org/10.1046/j.1471-4159.2002.01029.x
Guatimosim, Cristina, Court Hull, Henrique Von Gersdorff, and Marco A. M. Prado. “Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse.J Neurochem 82, no. 5 (September 2002): 1047–57. https://doi.org/10.1046/j.1471-4159.2002.01029.x.
Guatimosim C, Hull C, Von Gersdorff H, Prado MAM. Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse. J Neurochem. 2002 Sep;82(5):1047–57.
Guatimosim, Cristina, et al. “Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse.J Neurochem, vol. 82, no. 5, Sept. 2002, pp. 1047–57. Pubmed, doi:10.1046/j.1471-4159.2002.01029.x.
Guatimosim C, Hull C, Von Gersdorff H, Prado MAM. Okadaic acid disrupts synaptic vesicle trafficking in a ribbon-type synapse. J Neurochem. 2002 Sep;82(5):1047–1057.
Journal cover image

Published In

J Neurochem

DOI

ISSN

0022-3042

Publication Date

September 2002

Volume

82

Issue

5

Start / End Page

1047 / 1057

Location

England

Related Subject Headings

  • Synaptic Vesicles
  • Synaptic Transmission
  • Synapses
  • Retina
  • Quaternary Ammonium Compounds
  • Pyridinium Compounds
  • Patch-Clamp Techniques
  • Okadaic Acid
  • Neurology & Neurosurgery
  • In Vitro Techniques