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Cholinergic regulation of the sea urchin embryonic and larval development

Publication ,  Journal Article
Buznikov, GA; Bezuglov, VV; Nikitina, LA; Slotkin, TA; Lauder, JM
Published in: Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk
January 1, 2001

Choline esters of polyenoic fatty acids block cleavage divisions of sea urchins and evoke the formation of one-cell multinuclear embryos. If the fatty acids AA-Ch or DHA-Ch are added at the mid or late blastula stage, many cells are extruded, forming extra-embryonic cell clusters near the animal pole of embryos or larvae. Both effects are prevented by dimethylaminoethyl esters of polyenoic fatty acids (AA-DMAE or DHA-DMAE) or their 5-hydroxytryptamides. Nicotinic acetylcholine receptor antagonists, imechine, d-tubocurarine or QX-222 provide partial protection against AA-Ch or DHA-Ch. The organophosphate pesticide, chlorpyrifos, or a combination of (-)-nicotine + phorbol 12-myristate 13-acetate, also evoke the mass extrusion of transformed embryonic cells at the animal pole of larvae. These effects are similarly antagonized by AA-DMAE, DHA-DMAE, or fatty acids 5-hydroxytryptamides. Taking together, these results suggest that AA-Ch and DHA-Ch act on sea urchin embryos and larvae as agonists of acetylcholine receptors, whereas AA-DMAE and DHA-DMAE act as antagonists. The ability of fatty acids 5-hydroxytryptamides to prevent the effects of AA-Ch or DHA-Ch may be due to restoration of the normal dynamic balance of cholinergic and serotonergic signaling during cleavage divisions and gastrulation.

Duke Scholars

Published In

Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk

ISSN

0869-8139

Publication Date

January 1, 2001

Volume

87

Issue

11

Start / End Page

1548 / 1556

Related Subject Headings

  • 1116 Medical Physiology
 

Citation

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MLA
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Buznikov, G. A., Bezuglov, V. V., Nikitina, L. A., Slotkin, T. A., & Lauder, J. M. (2001). Cholinergic regulation of the sea urchin embryonic and larval development. Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova / Rossiiskaia Akademiia Nauk, 87(11), 1548–1556.
Buznikov, G. A., V. V. Bezuglov, L. A. Nikitina, T. A. Slotkin, and J. M. Lauder. “Cholinergic regulation of the sea urchin embryonic and larval development.” Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova / Rossiiskaia Akademiia Nauk 87, no. 11 (January 1, 2001): 1548–56.
Buznikov GA, Bezuglov VV, Nikitina LA, Slotkin TA, Lauder JM. Cholinergic regulation of the sea urchin embryonic and larval development. Rossiiskii fiziologicheskii zhurnal imeni IM Sechenova / Rossiiskaia akademiia nauk. 2001 Jan 1;87(11):1548–56.
Buznikov, G. A., et al. “Cholinergic regulation of the sea urchin embryonic and larval development.” Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova / Rossiiskaia Akademiia Nauk, vol. 87, no. 11, Jan. 2001, pp. 1548–56.
Buznikov GA, Bezuglov VV, Nikitina LA, Slotkin TA, Lauder JM. Cholinergic regulation of the sea urchin embryonic and larval development. Rossiiskii fiziologicheskii zhurnal imeni IM Sechenova / Rossiiskaia akademiia nauk. 2001 Jan 1;87(11):1548–1556.

Published In

Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk

ISSN

0869-8139

Publication Date

January 1, 2001

Volume

87

Issue

11

Start / End Page

1548 / 1556

Related Subject Headings

  • 1116 Medical Physiology