Abi3bp regulates cardiac progenitor cell proliferation and differentiation.

Journal Article (Journal Article)

RATIONALE: Cardiac progenitor cells (CPCs) are thought to differentiate into the major cell types of the heart: cardiomyocytes, smooth muscle cells, and endothelial cells. We have recently identified ABI family, member 3 (NESH) binding protein (Abi3bp) as a protein important for mesenchymal stem cell biology. Because CPCs share several characteristics with mesenchymal stem cells, we hypothesized that Abi3bp would similarly affect CPC differentiation and proliferation. OBJECTIVE: To determine whether Abi3bp regulates CPC proliferation and differentiation. METHODS AND RESULTS: In vivo, genetic ablation of the Abi3bp gene inhibited CPC differentiation, whereas CPC number and proliferative capacity were increased. This correlated with adverse recovery after myocardial infarction. In vitro, CPCs, either isolated from Abi3bp knockout mice or expressing an Abi3bp shRNA construct, displayed a higher proliferative capacity and, under differentiating conditions, reduced expression of both early and late cardiomyocyte markers. Abi3bp controlled CPC differentiation via integrin-β1, protein kinase C-ζ, and v-akt murine thymoma viral oncogene homolog. CONCLUSIONS: We have identified Abi3bp as a protein important for CPC differentiation and proliferation.

Full Text

Duke Authors

Cited Authors

  • Hodgkinson, CP; Gomez, JA; Payne, AJ; Zhang, L; Wang, X; Dal-Pra, S; Pratt, RE; Dzau, VJ

Published Date

  • December 5, 2014

Published In

Volume / Issue

  • 115 / 12

Start / End Page

  • 1007 - 1016

PubMed ID

  • 25296984

Pubmed Central ID

  • PMC4258122

Electronic International Standard Serial Number (EISSN)

  • 1524-4571

Digital Object Identifier (DOI)

  • 10.1161/CIRCRESAHA.115.304216


  • eng

Conference Location

  • United States