Microchemical analysis of selenium in otoliths of two West Virginia fishes captured near mountaintop removal coal mining operations.

Published

Journal Article

Otoliths, calcified inner ear structures, were collected from creek chubs (Semotilus atromaculatus) and green sunfish (Lepomis cyanellus) living in mountaintop mining-impacted and reference streams and analyzed for selenium (Se) content using laser ablation-inductively coupled mass spectrometry. Significant differences in otolith Se were found between the 2 fish species. Results from the present study suggest that a retrospective reconstruction of Se concentrations in muscle can be derived from Se concentrations in otoliths in creek chub but not green sunfish, exemplifying the importance of species differences when determining partitioning of Se among specific tissues. Green sunfish otoliths from all sites contained background (<1 μg/g) or low (1-4 μg/g) average concentrations of whole-otolith Se. In contrast, creek chub otoliths from the historically mined site contained much higher (≥5 μg/g) concentrations of Se than for the same species in the unmined site or for the green sunfish. These data suggest that body burdens of Se in fish can vary considerably over time and that both the timing of sampling and species choice could heavily influence Se assessments.

Full Text

Duke Authors

Cited Authors

  • Arnold, MC; Friedrich, LA; Lindberg, TT; Ross, M; Halden, NM; Bernhardt, E; Palace, VP; Di Giulio, RT

Published Date

  • May 2015

Published In

Volume / Issue

  • 34 / 5

Start / End Page

  • 1039 - 1044

PubMed ID

  • 25639549

Pubmed Central ID

  • 25639549

Electronic International Standard Serial Number (EISSN)

  • 1552-8618

International Standard Serial Number (ISSN)

  • 0730-7268

Digital Object Identifier (DOI)

  • 10.1002/etc.2885

Language

  • eng