Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue.

Published

Journal Article

We report the development of a combined confocal Raman spectroscopy (CRS) and optical coherence tomography (OCT) instrument (CRS-OCT) capable of measuring analytes in targeted biological tissues with sub-100-micron spatial resolution. The OCT subsystem was used to measure depth-resolved tissue morphology and guide the acquisition of chemically-specific Raman spectra. To demonstrate its utility, the instrument was used to accurately measure depth-resolved, physiologically-relevant concentrations of Tenofovir, a microbicide drug used to prevent the sexual transmission of HIV, in ex vivo tissue samples.

Full Text

Duke Authors

Cited Authors

  • Maher, JR; Chuchuen, O; Henderson, MH; Kim, S; Rinehart, MT; Kashuba, ADM; Wax, A; Katz, DF

Published Date

  • June 2015

Published In

Volume / Issue

  • 6 / 6

Start / End Page

  • 2022 - 2035

PubMed ID

  • 26114026

Pubmed Central ID

  • 26114026

Electronic International Standard Serial Number (EISSN)

  • 2156-7085

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/boe.6.002022

Language

  • eng