Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy.

Published

Journal Article (Review)

Many recently discovered noncoding RNAs do not fold into a single native conformation but sample many different conformations along their free-energy landscape to carry out their biological function. Here we review solution-state NMR techniques that measure the structural, kinetic and thermodynamic characteristics of RNA motions spanning picosecond to second timescales at atomic resolution, allowing unprecedented insights into the RNA dynamic structure landscape. From these studies a basic description of the RNA dynamic structure landscape is emerging, bringing new insights into how RNA structures change to carry out their function as well as applications in RNA-targeted drug discovery and RNA bioengineering.

Full Text

Duke Authors

Cited Authors

  • Bothe, JR; Nikolova, EN; Eichhorn, CD; Chugh, J; Hansen, AL; Al-Hashimi, HM

Published Date

  • October 28, 2011

Published In

Volume / Issue

  • 8 / 11

Start / End Page

  • 919 - 931

PubMed ID

  • 22036746

Pubmed Central ID

  • 22036746

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

International Standard Serial Number (ISSN)

  • 1548-7091

Digital Object Identifier (DOI)

  • 10.1038/nmeth.1735

Language

  • eng