Requirement of dendritic Akt degradation by the ubiquitin-proteasome system for neuronal polarity.

Published

Journal Article

Asymmetric distributions of activities of the protein kinases Akt and glycogen synthase kinase 3beta (GSK-3beta) are critical for the formation of neuronal polarity. However, the mechanisms underlying polarized regulation of this pathway remain unclear. In this study, we report that the instability of Akt regulated by the ubiquitin-proteasome system (UPS) is required for neuron polarity. Preferential distribution in the axons was observed for Akt but not for its target GSK-3beta. A photoactivatable GFP fused to Akt revealed the preferential instability of Akt in dendrites. Akt but not p110 or GSK-3beta was ubiquitinated. Suppressing the UPS led to the symmetric distribution of Akt and the formation of multiple axons. These results indicate that local protein degradation mediated by the UPS is important in determining neuronal polarity.

Full Text

Duke Authors

Cited Authors

  • Yan, D; Guo, L; Wang, Y

Published Date

  • July 31, 2006

Published In

Volume / Issue

  • 174 / 3

Start / End Page

  • 415 - 424

PubMed ID

  • 16864652

Pubmed Central ID

  • 16864652

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.200511028

Language

  • eng

Conference Location

  • United States