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Requirement of dendritic Akt degradation by the ubiquitin-proteasome system for neuronal polarity.

Publication ,  Journal Article
Yan, D; Guo, L; Wang, Y
Published in: J Cell Biol
July 31, 2006

Asymmetric distributions of activities of the protein kinases Akt and glycogen synthase kinase 3beta (GSK-3beta) are critical for the formation of neuronal polarity. However, the mechanisms underlying polarized regulation of this pathway remain unclear. In this study, we report that the instability of Akt regulated by the ubiquitin-proteasome system (UPS) is required for neuron polarity. Preferential distribution in the axons was observed for Akt but not for its target GSK-3beta. A photoactivatable GFP fused to Akt revealed the preferential instability of Akt in dendrites. Akt but not p110 or GSK-3beta was ubiquitinated. Suppressing the UPS led to the symmetric distribution of Akt and the formation of multiple axons. These results indicate that local protein degradation mediated by the UPS is important in determining neuronal polarity.

Duke Scholars

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

July 31, 2006

Volume

174

Issue

3

Start / End Page

415 / 424

Location

United States

Related Subject Headings

  • Ubiquitin
  • Rats
  • Proto-Oncogene Proteins c-akt
  • Protein Processing, Post-Translational
  • Proteasome Inhibitors
  • Proteasome Endopeptidase Complex
  • Phosphorylation
  • Phosphatidylinositol 3-Kinases
  • Leupeptins
  • Glycogen Synthase Kinase 3 beta
 

Citation

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MLA
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Yan, D., Guo, L., & Wang, Y. (2006). Requirement of dendritic Akt degradation by the ubiquitin-proteasome system for neuronal polarity. J Cell Biol, 174(3), 415–424. https://doi.org/10.1083/jcb.200511028
Yan, Dong, Li Guo, and Yizheng Wang. “Requirement of dendritic Akt degradation by the ubiquitin-proteasome system for neuronal polarity.J Cell Biol 174, no. 3 (July 31, 2006): 415–24. https://doi.org/10.1083/jcb.200511028.
Yan, Dong, et al. “Requirement of dendritic Akt degradation by the ubiquitin-proteasome system for neuronal polarity.J Cell Biol, vol. 174, no. 3, July 2006, pp. 415–24. Pubmed, doi:10.1083/jcb.200511028.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

July 31, 2006

Volume

174

Issue

3

Start / End Page

415 / 424

Location

United States

Related Subject Headings

  • Ubiquitin
  • Rats
  • Proto-Oncogene Proteins c-akt
  • Protein Processing, Post-Translational
  • Proteasome Inhibitors
  • Proteasome Endopeptidase Complex
  • Phosphorylation
  • Phosphatidylinositol 3-Kinases
  • Leupeptins
  • Glycogen Synthase Kinase 3 beta