Naked DNA delivered intraportally expresses efficiently in hepatocytes.

Journal Article

Naked plasmid DNA in hypertonic solutions was injected intraportally in mice whose hepatic veins were transiently occluded. High levels of luciferase expression and beta-galactosidase expression in 1% of the hepatocytes throughout the entire liver were achieved using 100 micrograms of the respective plasmid vector. Two days after the intraportal injection of 100 micrograms of pCMVGH, the mean hGH serum concentration was 65 ng/ml +/- 26 (n = 7) which is approximately 50-fold above normal baseline levels. These unprecedented levels of foreign gene expression from naked plasmid DNA document the ability of parenchymal cells in vivo to take up naked DNA following intravascular delivery.

Duke Authors

Cited Authors

  • Budker, V; Zhang, G; Knechtle, S; Wolff, JA

Published Date

  • July 1996

Published In

Volume / Issue

  • 3 / 7

Start / End Page

  • 593 - 598

PubMed ID

  • 8818646

Electronic International Standard Serial Number (EISSN)

  • 1476-5462

International Standard Serial Number (ISSN)

  • 0969-7128

Language

  • eng