Naked DNA delivered intraportally expresses efficiently in hepatocytes.
Publication
, Journal Article
Budker, V; Zhang, G; Knechtle, S; Wolff, JA
Published in: Gene Ther
July 1996
Naked plasmid DNA in hypertonic solutions was injected intraportally in mice whose hepatic veins were transiently occluded. High levels of luciferase expression and beta-galactosidase expression in 1% of the hepatocytes throughout the entire liver were achieved using 100 micrograms of the respective plasmid vector. Two days after the intraportal injection of 100 micrograms of pCMVGH, the mean hGH serum concentration was 65 ng/ml +/- 26 (n = 7) which is approximately 50-fold above normal baseline levels. These unprecedented levels of foreign gene expression from naked plasmid DNA document the ability of parenchymal cells in vivo to take up naked DNA following intravascular delivery.
Duke Scholars
Published In
Gene Ther
ISSN
0969-7128
Publication Date
July 1996
Volume
3
Issue
7
Start / End Page
593 / 598
Location
England
Related Subject Headings
- beta-Galactosidase
- Transfection
- Mice
- Luciferases
- Liver
- Injections
- Humans
- Gene Transfer Techniques
- Gene Expression
- Epidermal Growth Factor
Citation
APA
Chicago
ICMJE
MLA
NLM
Budker, V., Zhang, G., Knechtle, S., & Wolff, J. A. (1996). Naked DNA delivered intraportally expresses efficiently in hepatocytes. Gene Ther, 3(7), 593–598.
Budker, V., G. Zhang, S. Knechtle, and J. A. Wolff. “Naked DNA delivered intraportally expresses efficiently in hepatocytes.” Gene Ther 3, no. 7 (July 1996): 593–98.
Budker V, Zhang G, Knechtle S, Wolff JA. Naked DNA delivered intraportally expresses efficiently in hepatocytes. Gene Ther. 1996 Jul;3(7):593–8.
Budker, V., et al. “Naked DNA delivered intraportally expresses efficiently in hepatocytes.” Gene Ther, vol. 3, no. 7, July 1996, pp. 593–98.
Budker V, Zhang G, Knechtle S, Wolff JA. Naked DNA delivered intraportally expresses efficiently in hepatocytes. Gene Ther. 1996 Jul;3(7):593–598.
Published In
Gene Ther
ISSN
0969-7128
Publication Date
July 1996
Volume
3
Issue
7
Start / End Page
593 / 598
Location
England
Related Subject Headings
- beta-Galactosidase
- Transfection
- Mice
- Luciferases
- Liver
- Injections
- Humans
- Gene Transfer Techniques
- Gene Expression
- Epidermal Growth Factor