Lung organoids: current uses and future promise.

Published

Journal Article (Review)

Lungs are composed of a system of highly branched tubes that bring air into the alveoli, where gas exchange takes place. The proximal and distal regions of the lung contain epithelial cells specialized for different functions: basal, secretory and ciliated cells in the conducting airways and type II and type I cells lining the alveoli. Basal, secretory and type II cells can be grown in three-dimensional culture, with or without supporting stromal cells, and under these conditions they give rise to self-organizing structures known as organoids. This Review summarizes the different methods for generating organoids from cells isolated from human and mouse lungs, and compares their final structure and cellular composition with that of the airways or alveoli of the adult lung. We also discuss the potential and limitations of organoids for addressing outstanding questions in lung biology and for developing new drugs for disorders such as cystic fibrosis and asthma.

Full Text

Duke Authors

Cited Authors

  • Barkauskas, CE; Chung, M-I; Fioret, B; Gao, X; Katsura, H; Hogan, BLM

Published Date

  • March 15, 2017

Published In

Volume / Issue

  • 144 / 6

Start / End Page

  • 986 - 997

PubMed ID

  • 28292845

Pubmed Central ID

  • 28292845

Electronic International Standard Serial Number (EISSN)

  • 1477-9129

Digital Object Identifier (DOI)

  • 10.1242/dev.140103

Language

  • eng

Conference Location

  • England