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Profiling the neutralizing antibody response in chronically HIV-1 CRF07_BC-infected intravenous drug users naïve to antiretroviral therapy.

Publication ,  Journal Article
Hu, X; Hu, Y; Zhao, C; Gao, H; Greene, KM; Ren, L; Ma, L; Ruan, Y; Sarzotti-Kelsoe, M; Montefiori, DC; Hong, K; Shao, Y
Published in: Sci Rep
April 7, 2017

Characterizing neutralizing antibody (NAb) responses in individuals infected with diverse HIV-1 strains is necessary to reveal the novel targets for regional preventive and therapeutic strategies development. We evaluated the prevalence, breadth, and potency of NAb responses in 98 CRF07_BC-infected individuals using a large, multi-subtype panel of 30 tier 2-3 Env-pseudotyped viruses. Furthermore, we compared the neutralization pattern of CRF07_BC-infected people with that of subtype B'-infected individuals in China. Of the 98 plasma samples tested, 18% neutralized more than 80% of viruses in the panel, and 53% neutralized more than 50%, suggesting the presence of broadly NAbs in these individuals. A preferential intra-subtype neutralization of CRF07_BC was found. Notably, CRF07_BC-infected individuals generated higher neutralization titers against intra-subtype viruses than subtype B'-infected individuals with longer infection length. However, subtype B'-infected individuals mounted broader neutralization responses against inter-subtype viruses than CRF07_BC infection with shorter infection time, indicating the transition from narrow autologous to broad heterologous neutralization over time. Neutralization activity of the top six plasmas from each cohort was attributable to IgG fraction, and half of them developed CD4 binding site antibody reactivity. Heatmap analysis identified three statistically robust clusters of plasmas that offer valuable resources for further in-depth virological and immunological study.

Duke Scholars

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

April 7, 2017

Volume

7

Start / End Page

46308

Location

England

Related Subject Headings

  • Male
  • Humans
  • Hela Cells
  • HeLa Cells
  • HIV-1
  • HIV Infections
  • HEK293 Cells
  • Female
  • Drug Users
  • Antibodies, Neutralizing
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hu, X., Hu, Y., Zhao, C., Gao, H., Greene, K. M., Ren, L., … Shao, Y. (2017). Profiling the neutralizing antibody response in chronically HIV-1 CRF07_BC-infected intravenous drug users naïve to antiretroviral therapy. Sci Rep, 7, 46308. https://doi.org/10.1038/srep46308
Hu, Xintao, Yuanyuan Hu, Chunhong Zhao, Hongmei Gao, Kelli M. Greene, Li Ren, Liying Ma, et al. “Profiling the neutralizing antibody response in chronically HIV-1 CRF07_BC-infected intravenous drug users naïve to antiretroviral therapy.Sci Rep 7 (April 7, 2017): 46308. https://doi.org/10.1038/srep46308.
Hu, Xintao, et al. “Profiling the neutralizing antibody response in chronically HIV-1 CRF07_BC-infected intravenous drug users naïve to antiretroviral therapy.Sci Rep, vol. 7, Apr. 2017, p. 46308. Pubmed, doi:10.1038/srep46308.
Hu X, Hu Y, Zhao C, Gao H, Greene KM, Ren L, Ma L, Ruan Y, Sarzotti-Kelsoe M, Montefiori DC, Hong K, Shao Y. Profiling the neutralizing antibody response in chronically HIV-1 CRF07_BC-infected intravenous drug users naïve to antiretroviral therapy. Sci Rep. 2017 Apr 7;7:46308.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

April 7, 2017

Volume

7

Start / End Page

46308

Location

England

Related Subject Headings

  • Male
  • Humans
  • Hela Cells
  • HeLa Cells
  • HIV-1
  • HIV Infections
  • HEK293 Cells
  • Female
  • Drug Users
  • Antibodies, Neutralizing